Catecholaminergic neurotransmitters regulate migration and repopulation of immature human CD34+ cells through Wnt signaling
| Autor: | Menachem Rubinstein, Neta Netzer, Arnon Nagler, Herzel Ben-Hur, Alexander Kalinkovich, Shoham Shivtiel, Tsvee Lapidot, Asaf Spiegel, Aya Ludin, Igor B. Resnick, Yaara Azaria, Polina Goichberg, Izhar Hardan |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Myeloid Dopamine Immunology Cell Population Antigens CD34 Biology CD38 Receptors Dopamine Mice Norepinephrine Catecholamines Cell Movement Internal medicine medicine Animals Humans Immunology and Allergy Progenitor cell education Cell Proliferation Catecholaminergic Neurotransmitter Agents education.field_of_study Wnt signaling pathway Hematopoietic Stem Cells Cell biology Mice Inbred C57BL Wnt Proteins Haematopoiesis Endocrinology medicine.anatomical_structure Dopamine Agonists Signal Transduction |
| Zdroj: | Nature Immunology. 8:1123-1131 |
| ISSN: | 1529-2916 1529-2908 |
| Popis: | Catecholamines are important regulators of homeostasis, yet their functions in hematopoiesis are poorly understood. Here we report that immature human CD34+ cells dynamically expressed dopamine and beta2-adrenergic receptors, with higher expression in the primitive CD34+CD38(lo) population. The myeloid cytokines G-CSF and GM-CSF upregulated neuronal receptor expression on immature CD34+ cells. Treatment with neurotransmitters increased the motility, proliferation and colony formation of human progenitor cells, correlating with increased polarity, expression of the metalloproteinase MT1-MMP and activity of the metalloproteinase MMP-2. Treatment with catecholamines enhanced human CD34+ cell engraftment of NOD-SCID mice through Wnt signaling activation and increased cell mobilization and bone marrow Sca-1+c-Kit+Lin- cell numbers. Our results identify new functions for neurotransmitters and myeloid cytokines in the direct regulation of human and mouse progenitor cell migration and development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|