Therapeutic efficacy of a multi-epitope vaccine against Helicobacter pylori infection in BALB/c mice model
| Autor: | Xuhu Mao, Wei-Ying Zhou, Yun Shi, Weijun Zhang, Chao Wu, Quanming Zou, Haixia Li, Gang Guo |
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| Rok vydání: | 2009 |
| Předmět: |
CD4-Positive T-Lymphocytes
Bacterial Toxins Administration Oral Epitopes T-Lymphocyte Enterotoxin Epitope BALB/c Helicobacter Infections Enterotoxins Mice Adjuvants Immunologic Immunity Escherichia coli Animals Humans Immunity Mucosal Mice Inbred BALB C Vaccines Synthetic General Veterinary General Immunology and Microbiology biology Helicobacter pylori Escherichia coli Proteins Public Health Environmental and Occupational Health biology.organism_classification Virology Antibodies Bacterial Immunoglobulin A Vaccination Infectious Diseases Immunization Immunoglobulin G Immunology Bacterial Vaccines biology.protein Molecular Medicine Epitopes B-Lymphocyte Female Antibody |
| Zdroj: | Vaccine. 27(36) |
| ISSN: | 1873-2518 |
| Popis: | Epitope vaccine is a promising option for therapeutic vaccination against Helicobacter pylori (H. pylori) infection. In this study, we constructed a multi-epitope vaccine with five epitopes and mucosal adjuvant E. coli heat-labile enterotoxin B subunit (LTB) named HUepi-LTB and evaluated its therapeutic effect against H. pylori infection in BALB/c mice model. HUepi-LTB containing three Th epitopes from UreB and two B cell epitopes from UreB and HpaA was constructed and expressed in E. coli. Oral therapeutic immunization with HUepi-LTB significantly decreased H. pylori colonization compared with oral immunization with PBS, and the protection was correlated with antigen-specific CD4+ T cells and IgG and mucosal IgA antibody responses. This multi-epitope vaccine may be a promising vaccine candidate that may help to control H. pylori infection. |
| Databáze: | OpenAIRE |
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