Evaluation of the diagnostic performance of panfungal polymerase chain reaction assay in invasive fungal diseases
| Autor: | Yu‑Hua Ji, Zhen Zhao, Jia‑Bin Sun, Li Hua, Zhi‑Fang Xing, Guo‑Jun Cao |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
panfungal polymerase chain reaction Cancer Research Diagnostic methods medicine.diagnostic_test Bone marrow transplantation 030106 microbiology Pcr assay invasive fungal diseases General Medicine Articles Biology Timely diagnosis blood sample law.invention 03 medical and health sciences Immunology and Microbiology (miscellaneous) law Immunology medicine Blood culture Polymerase chain reaction early diagnosis |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-0981 |
| Popis: | Timely diagnosis of invasive fungal diseases (IFDs) is important, as delays in treatment initiation are associated with increased mortality rates. However, early diagnosis of IFDs in immunocompromised patients remains difficult. The conventional diagnostic methods currently used for IFDs are not sufficiently effective. Molecular tests, such as polymerase chain reaction (PCR)-based assays, have great potential to improve the early diagnosis of IFDs due to their sensitivity and specificity. In the present study, the diagnostic performance of panfungal PCR assays in IFD patients who received bone marrow transplantation was evaluated. The results suggested that panfungal PCR assay offered a quick and convenient guide for clinical decision-making by identifying higher numbers of fungal species in comparison with the conventional blood culture method. Furthermore, panfungal PCR assay exhibited a sensitivity of 93% and a specificity of 71% in the diagnosis of IFD patients based on the EORTC/MSG criteria. Thus, the present study concluded that the reported PCR-based method was effective and sensitive in early IFD diagnosis and should be integrated into clinical decision-making for the treatment of IFDs in the future. |
| Databáze: | OpenAIRE |
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