Stem cells (p63+) in keratinocyte cultures from human adult skin
| Autor: | L. M. Popescu, T. Regalia, Olga Simionescu, Eugen Radu, Doina Dumitrescu |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Keratinocytes Cell division Cellular homeostasis Cell Separation Biology Immunofluorescence Article Cell Adhesion medicine Humans Microscopy Phase-Contrast Cell adhesion Cells Cultured reproductive and urinary physiology Skin Collagen type medicine.diagnostic_test Stem Cells Cell Biology In vitro Cell biology medicine.anatomical_structure Microscopy Fluorescence embryonic structures Molecular Medicine Stem cell Keratinocyte Biomarkers Cell Division |
| Zdroj: | Journal of Cellular and Molecular Medicine. 6:593-598 |
| ISSN: | 1582-4934 1582-1838 |
| DOI: | 10.1111/j.1582-4934.2002.tb00457.x |
| Popis: | Epidermal stem cells (ESC) are responsible for maintaining skin cellular homeostasis, as they give rise to fast-dividing transit amplifying cells committed to terminal differentiation, while retaining their self-renewal capacity. However, no pure ESC cultures are available and no highly specific cytochemical marker was identified. We report here the experimental conditions allowing the selective enrichment in ESC, using cultured adult human keratinocytes. The main step was the selection of cells able to rapidly adhere to human collagen type IV in vitro. Thus, an increased proportion of putative ESC of about 65% was obtained, as demonstrated by p63 expression. |
| Databáze: | OpenAIRE |
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