Detection and molecular characterization of β-lactamase genes in clinical isolates of Gram-negative bacteria in Southern Ecuador
| Autor: | Sofía Genoveva Ochoa Astutillo, Alessio Ausili, Ana Paulina Arévalo Jaramillo, Zorayda Patricia Toledo Barrigas, Diana Yessenia Calva Delgado |
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| Rok vydání: | 2016 |
| Předmět: |
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Bacterial 0301 basic medicine Microbiology (medical) Gram-negative bacteria Genotype ?-Lactam resistance ?-Lactamase 030106 microbiology lcsh:QR1-502 β-Lactam resistance lcsh:Microbiology beta-Lactamases lcsh:Infectious and parasitic diseases Microbiology 03 medical and health sciences Enterobacteriaceae Disk Diffusion Antimicrobial Tests polycyclic compounds Humans lcsh:RC109-216 Genotypic characterization Gene β-Lactamase Medicine(all) Genetics Direct sequencing biology biochemical phenomena metabolism and nutrition Amplicon biology.organism_classification Phenotype Anti-Bacterial Agents 030104 developmental biology Infectious Diseases ESBL Ecuador Pseudomonadaceae |
| Zdroj: | Brazilian Journal of Infectious Diseases, Volume: 20, Issue: 6, Pages: 627-630, Published: DEC 2016 Repositorio Universidad Técnica Particular de Loja Universidad Técnica Particular de Loja instacron:UTPL Brazilian Journal of Infectious Diseases, Vol 20, Iss 6, Pp 627-630 (2016) |
| ISSN: | 1413-8670 |
| Popis: | This work performed a phenotypic and genotypic characterization of 79 clinical isolates of Enterobacteriaceae and Pseudomonadaceae collected in hospitals of Southern Ecuadorin 2013. Our results showed a high incidence of β-lactamases and ESBLs with blaTEM and blaCTX-M as the prevalent genes, respectively. By direct sequencing of PCR amplicons, the different β-lactamases and variants of the genes were also distinguished. Our results revealed a predominance of TEM-1 β-lactamase and the presence of different CTX-M variants with a prevalence of CTX-M-15. Two infrequent CTX-M variants in South America were also identified. To the best of our knowledge, this is one of the first studies describing the genetic characteristics of β-lactamases in Ecuador. Keywords: β-Lactam resistance, β-Lactamase, ESBL, Genotypic characterization |
| Databáze: | OpenAIRE |
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