Prostanoid signaling, localization, and expression of IP receptors in rat thick ascending limb cells
| Autor: | Richard L. Hébert, Odette Laneuville, Kevin D. Burns, Tim O’Connor, Chris Neville, L. N. Peterson |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Male
medicine.medical_specialty Physiology G protein Receptor expression Receptors Prostaglandin Gi alpha subunit Prostacyclin Biology Receptors Epoprostenol Pertussis toxin Rats Sprague-Dawley Mice Internal medicine Cyclic AMP medicine Animals Tissue Distribution RNA Messenger Virulence Factors Bordetella Receptor Protein kinase A Protein Kinase C Epoprostenol Rats Cell biology Arginine Vasopressin Enzyme Activation Endocrinology Pertussis Toxin Loop of Henle Prostaglandins Adenylate Cyclase Toxin Signal transduction Signal Transduction medicine.drug |
| Zdroj: | American Journal of Physiology-Renal Physiology. 275:F904-F914 |
| ISSN: | 1522-1466 1931-857X |
| Popis: | It is widely held that only one prostacyclin (IP) receptor exists that can couple to guanine stimulatory nucleotide binding proteins (Gs) leading to activation of adenyl cyclase. Although IP receptor mRNA is expressed in vascular arterial smooth muscle cells and platelets, with lower level expression in mature thymocytes, splenic lymphocytes, and megakaryocytes, there is no molecular evidence for IP receptor expression in renal epithelial cells. The purpose of the present study was to obtain molecular evidence for the expression and localization of the IP receptor and to study the signaling pathways of IP receptor in rat medullary thick ascending limb (MTAL). Biochemical studies showed that IP prostanoids do not increase cAMP in rat MTAL. However, in the presence of vasopressin, inhibition of cAMP formation by prostacyclin (PGI2) analogs is pertussis toxin sensitive and does not activate protein kinase C. In situ hybridization studies localized IP receptor mRNA expression to MTAL in the rat kidney outer medulla. The results of RT-PCR of freshly isolated RNA from MTAL, with primers specific for the mouse IP receptor cDNA, produced an amplification product of the correct predicted size that contained an expected Nco I endonuclease restriction site. We conclude that rat renal epithelial cells express the IP receptor, coupled to inhibition of cAMP production. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|