Another formula for calculating the gene change rate in real-time RT-PCR

Autor:	Weichang Chen, Jicheng Yang, Shujian Chang
Rok vydání:	2008
Předmět:	Genetics Models Genetic Reverse Transcriptase Polymerase Chain Reaction Absolute quantification Gene Expression RNA General Medicine Biology Real-time polymerase chain reaction Gene expression Humans RNA Messenger HT29 Cells Molecular Biology Gene Algorithms
Zdroj:	Molecular Biology Reports. 36:2165-2168
ISSN:	1573-4978 0301-4851
DOI:	10.1007/s11033-008-9430-1
Popis:	Relative quantification real-time PCR has become a routine method in molecular biology research to study the small amount gene expression. There are a few mathematical models for relative quantification data analysis in real-time PCR, and the formula 2(-DDCT ) is one of the most frequently used method. In this paper, we are to present another equation which directly calculates the change rate (R (c)) of gene expression, and give a example to compare the application of this model with 2(-DDCT).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dcf9fa9eb6ac76dbbaf1c865a78644ae https://doi.org/10.1007/s11033-008-9430-1 Zobrazit plný text záznamu Full text from SpringerLink