Gut microbiota composition in obese and non-obese adult relatives from the highlands of Papua New Guinea
Autor: | Wataru Suda, Paul F. Horwood, Geraldine Masiria, Lorry Wawae, William Pomat, Marinjho E Jonduo, Masahiro Umezaki, Masahira Hattori, Andrew R. Greenhill, Mohammad Yazid Abdad, Lena Takayasu |
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Rok vydání: | 2020 |
Předmět: |
Adult
Male Operational taxonomic unit Firmicutes Zoology Gut flora Microbiology Feces Papua New Guinea 03 medical and health sciences RNA Ribosomal 16S Genetics medicine Prevotella Humans Obesity Microbiome Molecular Biology 030304 developmental biology 0303 health sciences Bacteria biology 030306 microbiology Bacteroidetes Biodiversity biology.organism_classification medicine.disease Gastrointestinal Microbiome Case-Control Studies Female |
Zdroj: | FEMS Microbiology Letters. 367 |
ISSN: | 1574-6968 |
Popis: | Obesity is a condition that results from an imbalance between energy intake and expenditure. Recently, obesity has been linked to differences in the composition of gut microbiota. To examine this association in Papua New Guinea (PNG) highlanders, fecal samples were collected from 18 adults; nine obese participants were paired with their non-obese relative. Amplification of the 16S rRNA gene targeting the V1–V2 region was performed on DNA extracts for each participant, with high-quality sequences selected and used for operational taxonomic unit clustering. The data showed Firmicutes and Bacteroidetes were the two dominant phyla, while at genus level Prevotella was the most dominant genus in all of the samples. Nonetheless, statistical evaluation of potential association between nutritional status and bacterial abundance at both phyla and genus levels showed no significant difference. Further studies, ideally in both rural and urban areas, are needed to evaluate the role of the gut microbiome in the occurrence of obesity in PNG and other resource-limited settings. |
Databáze: | OpenAIRE |
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