Influence of laminin substratum on cell proliferation and CALC I gene expression in medullary thyroid carcinoma C cell lines
| Autor: | N. Jeanne, E. Pidoux, F. Lekmine, F. Treilhou-Lahille, N. Segond, Bernard A. Roos, S. Lausson |
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| Rok vydání: | 2000 |
| Předmět: |
Calcitonin
medicine.medical_specialty Calcitonin Gene-Related Peptide Calcitonin gene-related peptide Biochemistry Extracellular matrix Mice Endocrinology Laminin Internal medicine medicine Tumor Cells Cultured Animals Humans Protein Isoforms Secretion RNA Messenger Thyroid Neoplasms Molecular Biology Basement membrane biology Cell growth Molecular biology Rats medicine.anatomical_structure Cell culture Carcinoma Medullary biology.protein Cell Division |
| Zdroj: | Molecular and cellular endocrinology. 157(1-2) |
| ISSN: | 0303-7207 |
| Popis: | Medullary thyroid carcinoma (MTC) originates from C cells, which secrete calcitonin (CT) and CT gene-related peptide (CGRP), the two splice peptide products of the CALC I gene. Normal and hyperplastic C cells are intrafollicular, in contact with the basement membrane (BM) that is maintained around the differentiated tumors. To investigate the relationships between MTC evolution and BM constituents, we examined the modifications induced by laminin-1 and -2 (merosin), two isoforms colocalized in the follicular BM, on three MTC cell lines: murine rMTC 6-23 and CA-77 cells, and human TT cells. Laminin exerted a mitogenic activity on rMTC 6-23 and on TT cells, causing a concurrent decrease in both CT and CGRP mRNA levels and production of the peptides. Conversely, laminin reduced the proliferation rate and enhanced CGRP synthesis and secretion in CA-77 cells. This antiproliferative response, which coincides with an increase in differentiation markers, is comparable to that reported in normal cells and also in the neoplastic Caco-2 cell line. This suggests that laminin could exert opposite effects depending on the stage of tumor evolution. |
| Databáze: | OpenAIRE |
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