Expression of tumor necrosis factor-alpha–converting enzyme and tumor necrosis factor-alpha in human myocarditis
| Autor: | Hidenori Saitoh, Hidetoshi Satoh, Motoyuki Nakamura, Katsuhiko Hiramori, Mamoru Satoh, Ikuo Segawa |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Myocarditis Adolescent medicine.medical_treatment Biopsy ADAM17 Protein Pathogenesis Medicine Humans RNA Messenger Aged Metalloproteinase medicine.diagnostic_test business.industry Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha Myocardium Metalloendopeptidases Middle Aged medicine.disease Prognosis ADAM Proteins Cytokine Real-time polymerase chain reaction Disease Progression Immunohistochemistry Tumor necrosis factor alpha Female business DNA Probes Cardiology and Cardiovascular Medicine Biomarkers |
| Zdroj: | Journal of the American College of Cardiology. (4):1288-1294 |
| ISSN: | 0735-1097 |
| DOI: | 10.1016/S0735-1097(00)00827-5 |
| Popis: | OBJECTIVES We determined whether tumor necrosis factor-alpha-converting enzyme (TACE) is expressed with tumor necrosis factor-alpha (TNF-alpha) in myocarditis. BACKGROUND Tumor necrosis factor-alpha-converting enzyme, which has recently been identified as belonging to the family of metalloproteinase disintegrin proteins, is responsible for the conversion of TNF-alpha precursor to its mature form. METHODS We examined TACE and TNF-alpha expressions in endomyocardial biopsy tissues obtained from 14 patients with myocarditis and five control subjects by using quantitative reverse transcriptase polymerase chain reaction and immunohistochemistry. RESULTS Expression of TNF-alpha and TACE messenger ribonucleic acid (mRNA) was significantly greater in the myocarditis group than in the control group. A positive correlation was found between TNF-alpha and TACE mRNAs (r = 0.83, p < 0.05). Six patients with severe myocarditis underwent repeat biopsies. Although TNF-alpha and TACE mRNAs were expressed at high levels in the initial biopsies, a marked decrease was noted in the repeat biopsies. The immunostainings for TNF-alpha and TACE were positive in the myocytes and interstitial cells of myocardium obtained from patients with myocarditis. Expression of TACE and TNF-alpha mRNAs was greater in the subgroup in New York Heart Association functional class III or IV than in the subgroup in class I or II. Expression of TACE and TNF-alpha mRNA was correlated positively with left ventricular volume (TNF-alpha: r = 0.85; TACE: r = 0.80) and negatively with left ventricular systolic function (TNF-alpha: r = -0.85; TACE: r = -0.85). CONCLUSIONS These findings indicate that the expression of TNF-alpha and TACE may have important implications in the pathogenesis of myocarditis and may influence advanced cardiac dysfunction in myocarditis. |
| Databáze: | OpenAIRE |
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