Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells
| Autor: | Tomáš Riedel, Julia Pajorova, Eduard Brynda, Lucie Bacakova, Margit Zaloudkova, František Lopot, Daniel Hadraba, Denisa Stranska, Marketa Bacakova |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
collagen
Keratinocytes Materials science Polymers Biophysics Nanofibers Pharmaceutical Science Bioengineering Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Fibrin Biomaterials Extracellular matrix chemistry.chemical_compound Polylactic acid Tissue engineering International Journal of Nanomedicine Tensile Strength Drug Discovery Cell Adhesion Humans skin-tissue engineering Cells Cultured Original Research nanocoating Cell Proliferation biology Tissue Engineering Tissue Scaffolds Organic Chemistry General Medicine Adhesion Fibroblasts 021001 nanoscience & nanotechnology skin cells 0104 chemical sciences Extracellular Matrix Fibronectins Fibronectin Membrane chemistry Nanofiber biology.protein 0210 nano-technology |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 1176-9114 |
| Popis: | Marketa Bacakova,1,2 Julia Pajorova,1,2 Denisa Stranska,3 Daniel Hadraba,1,4 Frantisek Lopot,4 Tomas Riedel,5 Eduard Brynda,5 Margit Zaloudkova,6 Lucie Bacakova1 1Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, 2Second Faculty of Medicine,Charles University, Prague, 3InStar Technologies, Liberec, 4Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport, Charles University, 5Department of Chemistry and Physics of Surfaces and Biointerfaces, Institute of Macromolecular Chemistry, 6Department of Composites and Carbon Materials, Institute of Rock Structure and Mechanics, Czech Academy of Sciences, Prague, Czech Republic Abstract: Protein-coated resorbable synthetic polymeric nanofibrous membranes are promising for the fabrication of advanced skin substitutes. We fabricated electrospun polylactic acid and poly(lactide-co-glycolic acid) nanofibrous membranes and coated them with fibrin or collagen I. Fibronectin was attached to a fibrin or collagen nanocoating, in order further to enhance the cell adhesion and spreading. Fibrin regularly formed a coating around individual nanofibers in the membranes, and also formed a thin noncontinuous nanofibrous mesh on top of the membranes. Collagen also coated most of the fibers of the membrane and randomly created a soft gel on the membrane surface. Fibronectin predominantly adsorbed onto a thin fibrin mesh or a collagen gel, and formed a thin nanofibrous structure. Fibrin nanocoating greatly improved the attachment, spreading, and proliferation of human dermal fibroblasts, whereas collagen nanocoating had a positive influence on the behavior of human HaCaT keratinocytes. In addition, fibrin stimulated the fibroblasts to synthesize fibronectin and to deposit it as an extracellular matrix. Fibrin coating also showed a tendency to improve the ultimate tensile strength of the nanofibrous membranes. Fibronectin attached to fibrin or to a collagen coating further enhanced the adhesion, spreading, and proliferation of both cell types. Keywords: skin-tissue engineering, nanocoating, nanofibers, skin cells, fibrin, collagen |
| Databáze: | OpenAIRE |
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