Zymographic patterns of MMP-2 and MMP-9 in the CSF and cerebellum of dogs with subacute distemper leukoencephalitis
| Autor: | Érica de Souza Ribeiro, Andressa A. Machado, Guilherme D. Melo, Gisele Fabrino Machado, Olívia C. Moraes, Milena Sato de Souza, Cáris Maroni Nunes, Daniela S. Migliolo |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista Júlio de Mesquita Filho = São Paulo State University (UNESP) |
| Rok vydání: | 2012 |
| Předmět: |
Canine distemper virus
Immunology Central nervous system Biology Matrix metalloproteinase Blood–brain barrier Gene Expression Regulation Enzymologic Article 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Dogs Leukoencephalopathies Cerebellum medicine Demyelinating disease Animals Dog Diseases Distemper ComputingMilieux_MISCELLANEOUS 030304 developmental biology Inflammation 0303 health sciences General Veterinary Microglia Canine distemper Zymography medicine.disease 3. Good health Myelin basic protein medicine.anatomical_structure Matrix metalloproteinases Matrix Metalloproteinase 9 [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology biology.protein Matrix Metalloproteinase 2 030217 neurology & neurosurgery |
| Zdroj: | Veterinary Immunology and Immunopathology Veterinary Immunology and Immunopathology, 2013, 154 (1-2), pp.68-74. ⟨10.1016/j.vetimm.2013.04.006⟩ |
| ISSN: | 1873-2534 0165-2427 |
| DOI: | 10.1016/j.vetimm.2013.04.006⟩ |
| Popis: | Distemper leukoencephalitis is a disease caused by the canine distemper virus (CDV) infection. It is a demyelinating disease affecting mainly the white matter of the cerebellum and areas adjacent to the fourth ventricle; the enzymes of the matrix metalloproteinases (MMPs) group, especially MMP-2 and MMP-9 have a key role in the myelin basic protein fragmentation and in demyelination, as well as in leukocyte traffic into the nervous milieu. To evaluate the involvement of MMPs during subacute distemper leukoencephalitis, we measured the levels of MMP-2 and MMP-9 by zymography in the cerebrospinal fluid (CSF) and in the cerebellum of 14 dogs naturally infected with CDV and 10 uninfected dogs. The infected dogs presented high levels of pro-MMP-2 in the CSF and elevated levels of pro-MMP-2 and pro-MMP-9 in the cerebellar tissue. Active MMP-2 was detected in the CSF of some infected dogs. As active MMP-2 and MMP-9 are required for cellular migration across the blood-brain barrier and any interference between MMPs and their inhibitors may result in an amplification of demyelination, this study gives additional support to the involvement of MMPs during subacute distemper leukoencephalitis and suggests that MMP-2 and MMP-9 may take part in the brain inflammatory changes of this disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect