The irradiation of hepatocytes with He-Ne laser causes an increase of cytosolic free calcium concentration and an increase of cell membrane potential, correlated with it, both increases taking place in an oscillatory manner
| Autor: | Rosa Anna Vacca, Margherita Greco, Salvatore Passarella, F. Fontana, V.A. Petragallo, L. Moro |
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| Předmět: |
Male
Light Liver cytology Clinical Biochemistry chemistry.chemical_element Neon Calcium Helium Biochemistry Fluence Membrane Potentials Cytosol Biological Clocks Genetics medicine Animals Irradiation Rats Wistar Molecular Biology Calcium metabolism Membrane potential Lasers Cell Membrane Cell Biology Rats Cell biology medicine.anatomical_structure Liver chemistry Hepatocyte |
| Zdroj: | Scopus-Elsevier |
| Popis: | Isolated hepatocytes were irradiated with Helium-Neon laser (fluence: 0.24 Joules x cm-2, fluence rate: 12 mW x cm-2) and changes of both cytosolic free Ca2+ concentration and cell membrane potential were checked by measuring fura-2 and bis-oxonol fluorescence respectively. Irradiation resulted in an enhancement in cytosolic free Ca2+ concentration that requires the presence of Ca2+ in the phase outside hepatocytes; consistently an increase in cell membrane potential was measured correlated with it. Interestingly, the rate of increase of both cytosolic free Ca2+ concentration and cell membrane potential shows special time dependent features similar to those peculiar of oscillatory processes. |
| Databáze: | OpenAIRE |
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