Ex vivo innate responses to particulate matter from livestock farms in asthma patients and healthy individuals

Autor: de Groot, Linsey E S, Liu, Dingyu, Dierdorp, Barbara S, Fens, Niki, van de Pol, Marianne A, Sterk, Peter J, Kulik, Wim, Gerlofs-Nijland, Miriam E, Cassee, Flemming R, Pinelli, Elena, Lutter, René, One Health Toxicologie, Sub Cond-Matter Theory, Stat & Comp Phys, Dep IRAS, IRAS OH Toxicology, dIRAS RA-1
Přispěvatelé: One Health Toxicologie, Sub Cond-Matter Theory, Stat & Comp Phys, Dep IRAS, IRAS OH Toxicology, dIRAS RA-1, Graduate School, AII - Inflammatory diseases, Pulmonology, Laboratory Genetic Metabolic Diseases, Amsterdam Gastroenterology Endocrinology Metabolism
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Farms
Livestock
Health
Toxicology and Mutagenesis
medicine.medical_treatment
Sus scrofa
Inflammation
Leukocytes
Mononuclear/chemistry
medicine.disease_cause
Peripheral blood mononuclear cell
Superoxide dismutase
lcsh:RC963-969
03 medical and health sciences
0302 clinical medicine
Particulate Matter/adverse effects
medicine
Leukocytes
Animals
Mononuclear/chemistry
030212 general & internal medicine
Respiratory system
Asthma
Air Pollutants
biology
business.industry
lcsh:Public aspects of medicine
Research
Goats
Public Health
Environmental and Occupational Health
lcsh:RA1-1270
medicine.disease
Oxidative Stress
Cytokine
030228 respiratory system
Peripheral blood mononuclear cells
Immunology
lcsh:Industrial medicine. Industrial hygiene
biology.protein
Leukocytes
Mononuclear
Cytokines
Tumor necrosis factor alpha
medicine.symptom
business
Particulate matter
Chickens
Environmental Health
Oxidative stress
Zdroj: Environmental Health: A Global Access Science Source, 19(1). BioMed Central
Environmental Health
Environmental health, 19(1):78. BioMed Central
Environmental Health, Vol 19, Iss 1, Pp 1-10 (2020)
ISSN: 1476-069X
DOI: 10.1186/s12940-020-00632-8
Popis: Background Asthma patients suffer from periodic acute worsening of symptoms (i.e. loss of asthma control or exacerbations), triggered by a variety of exogenous stimuli. With the growing awareness that air pollutants impact respiratory diseases, we investigated whether particulate matter (PM) derived from various livestock farms (BioPM) differentially affected innate and oxidative stress responses in asthma and health. Methods Peripheral blood mononuclear cells (PBMCs), collected from patients sequentially before and during loss of asthma control and from healthy individuals, were exposed to BioPM collected from chicken, goat and pig farms (1 and 5 μg/ml), with or without pre-treatment with antioxidants. Cytokine release and oxidative stress were assessed. Results PBMCs produced IFNγ, IL-1β, IL-10 and TNFα upon stimulation with BioPM, with that from pig farms inducing the highest cytokine levels. Overall, cytokine production was irrespective of the presence or state of disease. However, PBMCs from stable asthma patients upon exposure to the three BioPM showed more extreme TNFα responses than those from healthy subjects. Furthermore, PBMCs obtained during loss of asthma control that were exposed to BioPM from pig farms showed enhanced IFNγ release as well as decreased oxidative stress levels upon pre-treatment with N-acetylcysteine (NAC) compared to stable disease. NAC, but not superoxide dismutase and catalase, also counteracted BioPM-induced cytokine release, indicating the importance of intracellular reactive oxygen species in the production of cytokines. Conclusions BioPM triggered enhanced pro-inflammatory responses by PBMCs from both healthy subjects and asthma patients, with those from patients during loss of asthma control showing increased susceptibility to BioPM from pig farms in particular.
Databáze: OpenAIRE
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