Human respiratory syncytial virus (hRSV) RNA quantification in nasopharyngeal secretions identifies the hRSV etiologic role in acute respiratory tract infections of hospitalized infants
| Autor: | Antonietta Marchi, Alice Bertaina, Elena Percivalle, Giuseppe Gerna, Mauro Stronati, Francesca Rovida, Fausto Baldanti, Giulia Campanini |
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| Rok vydání: | 2007 |
| Předmět: |
Paramyxoviridae
RT-PCR Respiratory Syncytial Virus Infections Respiratory syncytial virus Sensitivity and Specificity Article Pneumovirinae Nasopharynx Virology medicine Humans Viral respiratory infections Mononegavirales Respiratory Tract Infections DFA direct fluorescent antibody Acute respiratory tract infection Respiratory tract infections biology Reverse Transcriptase Polymerase Chain Reaction ARTI acute respiratory tract infection Infant Pneumovirus biology.organism_classification hRSV RNA quantification Hospitalization hRSV human respiratory syncytial virus NPA nasopharyngeal aspirate Infectious Diseases medicine.anatomical_structure Respiratory Syncytial Virus Human Acute Disease Immunology RNA Viral CC cell culture RT-PCR reverse transcription-PCR Viral load Respiratory tract |
| Zdroj: | Journal of Clinical Virology |
| ISSN: | 1386-6532 |
| DOI: | 10.1016/j.jcv.2007.03.009 |
| Popis: | Background Human respiratory syncytial virus (hRSV) detection in nasopharyngeal aspirates (NPAs) from infants with acute respiratory tract infection (ARTI) does not prove the hRSV etiology of the current ARTI episode. HRSV RNA quantification may help in affording this issue. Objectives hRSV was detected by quantitative reverse transcription-PCR in NPAs taken upon admission to hospital and, whenever possible, at discharge and subsequent medical visits. Study design Prospective study, including 63 infants affected by either hRSV upper or lower ARTI. Results Based on the kinetics of viral load, hRSV etiology was identified in 25 infants in whom hRSV load dropped from 2.5 × 106 upon admission (presence of respiratory symptoms) to 7.5 × 102 RNA copies/ml NPA upon discharge (absence of symptoms) after a median time of 5 days, and in 19 infants, in whom hRSV load was determined at admission only, in association with clinical symptoms (2.4 × 106 copies/ml). Furthermore, low levels of hRSV RNA ( |
| Databáze: | OpenAIRE |
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