Effects of Quaternary Ammonium Silane Coatings on Mixed Fungal and Bacterial Biofilms on Tracheoesophageal Shunt Prostheses
| Autor: | Janine J. H. Oosterhof, Henny C. van der Mei, Bernard F. A. M. van der Laan, Henk J. Busscher, Kevin J. D. A. Buijssen |
|---|---|
| Přispěvatelé: | Faculteit Medische Wetenschappen/UMCG, Damage and Repair in Cancer Development and Cancer Treatment (DARE), Man, Biomaterials and Microbes (MBM), Personalized Healthcare Technology (PHT) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Antifungal Agents
Surface Properties Biocompatible Materials Public Health Microbiology engineering.material Silicone rubber Applied Microbiology and Biotechnology Chloride Microbiology chemistry.chemical_compound Esophagus Coating Yeasts medicine Humans Ammonium ANTIMICROBIAL ACTIVITY CANDIDA TARGETED OLIGONUCLEOTIDE PROBES Microscopy Confocal Ecology biology Bacteria Biofilm IN-VITRO Silanes biology.organism_classification Silane Yeast Anti-Bacterial Agents Quaternary Ammonium Compounds Trachea VOICE PROSTHESES chemistry INFECTIONS Biofilms engineering Silicone Elastomers POPULATIONS Larynx Artificial Food Science Biotechnology medicine.drug Nuclear chemistry |
| Zdroj: | Applied and environmental microbiology, 72(5), 3673-3677. AMER SOC MICROBIOLOGY |
| ISSN: | 0099-2240 |
| Popis: | Two quaternary ammonium silanes (QAS) were used to coat silicone rubber tracheoesophageal shunt prostheses, yielding a positively charged surface. One QAS coating [(trimethoxysilyl)-propyldimethyloctadecylammonium chloride] was applied through chemical bonding, while the other coating, Biocidal ZF, was sprayed onto the silicone rubber surface. The sprayed coating lost its stability within an hour, while the chemically bonded coating appeared stable. Upon incubation in an artificial throat model, allowing simultaneous adhesion and growth of yeast and bacteria, all coated prostheses showed significant reductions in the numbers of viable yeast (to 12% to 16%) and bacteria (to 27% to 36%) compared with those for silicone rubber controls, as confirmed using confocal laser scanning microscopy after live/dead staining of the biofilms. In situ hybridization with fluorescently labeled oligonucleotide probes showed that yeasts expressed hyphae on the untreated and Biocidal ZF-coated prostheses but not on the QAS-coated prostheses. Whether this is a result of the positive QAS coating or is due to the reduced number of bacteria is currently unknown. In summary, this is the first report on the inhibitory effects of positively charged coatings on the viability of yeasts and bacteria in mixed biofilms. Although the study initially aimed at reducing voice prosthetic biofilms, its relevance extends to all biomedical and environmental surfaces where mixed biofilms develop and present a problem. |
| Databáze: | OpenAIRE |
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