Multiplex PCRs for Identification of Escherichia coli Virulence Genes
| Autor: | R. Odedra, M. A. Pass, R. M. Batt |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Microbiology (medical)
Virulence Biology medicine.disease_cause Polymerase Chain Reaction law.invention Microbiology Feces Dogs law Multiplex polymerase chain reaction Escherichia coli medicine Animals Multiplex Gene Polymerase chain reaction DNA Primers Electrophoresis Agar Gel Genetics Bacteriology biology.organism_classification Enterobacteriaceae Bacteria |
| Zdroj: | Journal of Clinical Microbiology. 38:2001-2004 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.38.5.2001-2004.2000 |
| Popis: | PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs. |
| Databáze: | OpenAIRE |
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