Annona muricata Leaf Extract Triggered Intrinsic Apoptotic Pathway to Attenuate Cancerous Features of Triple Negative Breast Cancer MDA-MB-231 Cells
Autor: | Sait Byul Park, Hyeri Jang, Jee Young Kim, Kwangho Song, Min Kyoung Park, Yeong Shik Kim, Thien T. P. Dao, Soo Ueng Gan |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
biology Article Subject Chemistry Annonacin Motility lcsh:Other systems of medicine biology.organism_classification lcsh:RZ201-999 In vitro 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Complementary and alternative medicine Apoptosis Cell culture 030220 oncology & carcinogenesis Cancer research Viability assay skin and connective tissue diseases Annona muricata Triple-negative breast cancer Research Article |
Zdroj: | Evidence-based Complementary and Alternative Medicine : eCAM Evidence-Based Complementary and Alternative Medicine, Vol 2018 (2018) |
ISSN: | 1741-427X |
DOI: | 10.1155/2018/7972916 |
Popis: | Annona muricataL., known as graviola, is an evergreen plant of the tropical regions and is a rich source of natural products. Graviola has various biological activities, and it is best known for its anticancer activity. This study aimed to investigate the effects of crude graviola extractin vitroon breast cancer cells; in particular, we aimed to identify an agent against triple negative breast cancer (TNBC). We used the TNBC MDA-MB-231 cell line as the experimental model and the ER(+) non-TNBC MCF-7 breast cancer cell line as the control. We identified annonaceous acetogenins, including annonacin isomers, characteristic to this plant by using liquid chromatography tandem mass spectrometry (LC/MS/MS). We observed a significant decrease in the cell viability in both cell lines within 48 h, whereas impaired cell motility and invasiveness were observed only in the MDA-MB-231 cell line. While the MCF-7 cells showed an ER-dependent mechanism of apoptosis, the apoptosis of MDA-MB-231 cells was governed by an intrinsic apoptotic pathway triggered by graviola leaf extract (GLE). |
Databáze: | OpenAIRE |
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