Kinetic analysis of 3H-serotonin accumulation in four regions of rat brain after lesions in the medial forebrain bundle
| Autor: | Libby M. Yunger, John A. Harvey |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Male
Serotonin medicine.medical_specialty Serotonin uptake Dopamine Hippocampus Serotonergic General Biochemistry Genetics and Molecular Biology Norepinephrine Cerebellum Internal medicine medicine Animals Diencephalon General Pharmacology Toxicology and Pharmaceutics Medial forebrain bundle Cerebral Cortex Neocortex Chemistry Temperature Brain Biological Transport General Medicine Rats Kinetics Endocrinology medicine.anatomical_structure nervous system Catecholaminergic cell groups Caudate Nucleus Neuroscience Synaptosomes medicine.drug |
| Zdroj: | Life Sciences. 19:105-115 |
| ISSN: | 0024-3205 |
| Popis: | Kinetic analysis of 3 H-serotonin accumulation by crude synaptosomal suspensions of neocortex, hippocampus and caudate or by whole homogenates of cerebellum revealed the presence of a high affinity uptake component having an apparent K m for serotonin which ranged from 2.8 to 6.0 × 10 −8 M. A second, low affinity, uptake component with an apparent K m of 7 × 10 −6 M was present in caudate. A comparable low affinity uptake component for serotonin was not observed in neocortex, hippocampus or cerebellum. Lesions in the medial forebrain bundle produced significant decreases in serotonin comtent of neocortes, hippocampus and caudate (66 to 75%) and a significant increase in serotonin content of cerebellum (25%). The lesions did not affect the apparent K m of the high affinity uptake system but did produce change in V max which paralleled the changes in content of serotonin. The lesions also produced decreases in dopamine and norepinephrine content of caudate and a comparable decrease in the V max of the low affinity uptake system with no change in the apparent K m . There was a correlation of 0.97 between the endogenous content of serotonin and the V max of the high affinity uptake system. These results support the view that the high and low affinity components of serotonin uptake represent accumulation into serotonergic and catecholaminergic neurons, respectively. |
| Databáze: | OpenAIRE |
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