Designing siRNA-conjugated plant oil-based nanoparticles for gene silencing and cancer therapy

Autor: Ebru Kilicay, Baki Hazer, Nur Merve Anilmis, Goknur Kara, Emir Baki Denkbaş
Přispěvatelé: Zonguldak Bülent Ecevit Üniversitesi, Kapadokya Üniversitesi
Rok vydání: 2019
Předmět:
Lung Neoplasms
Linoleic acid
Cancer therapy
Pharmaceutical Science
Nanoparticle
Bioengineering
Adenocarcinoma of Lung
02 engineering and technology
Nanoconjugates
Conjugated system
030226 pharmacology & pharmacy
Styrene
Polyethylene Glycols
Linoleic Acid
PS-g-PLina-PEG
03 medical and health sciences
chemistry.chemical_compound
PS-g-PLina
0302 clinical medicine
Colloid and Surface Chemistry
medicine
Gene silencing
Humans
Plant Oils
Physical and Theoretical Chemistry
RNA
Small Interfering
Drug Carriers
Organic Chemistry
technology
industry
and agriculture
respiratory system
021001 nanoscience & nanotechnology
medicine.disease
Epithelium
respiratory tract diseases
lung cancer
medicine.anatomical_structure
RNAi Therapeutics
chemistry
A549 Cells
siRNA
Cancer research
Adenocarcinoma
Polystyrenes
nanoparticles
0210 nano-technology
Zdroj: Journal of microencapsulation. 36(7)
ISSN: 1464-5246
Popis: In this study, the anticancer activities of two siRNA carriers were compared using a human lung adenocarcinoma epithelial cell line (A549). Firstly, poly(styrene)-graft-poly(linoleic acid) (PS-g-PLina) and poly(styrene)-graft-poly(linoleic acid)-graft-poly(ethylene glycol) (PS-g-PLina-g-PEG) graft copolymers were synthesized by free-radical polymerization. PS-PLina and PS-PLina-PEG nanoparticles (NPs) were prepared by solvent evaporation method and were then characterized. The size was found as 150 ± 10 nm for PS-PLina and 184 ± 6 nm for PS-PLina-PEG NPs. The NPs were functionalized with poly(l-lysine) (PLL) for c-myc siRNA conjugation. siRNA entrapment efficiencies were found in the range of 4–63% for PS-PLina-PLL and 6–42% for PS-PLina-PEG-PLL NPs. The short-term stability test was realised for 1 month. siRNA release profiles were also investigated. In vitro anticancer activity of siRNA-NPs was determined by MTT, flow cytometry, and fluorescence microscopy analyses. Obtained findings showed that both NPs systems were promising as siRNA delivery tool for lung cancer therapy. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
BAGP-001, BEU-2016, 33496813-01
This work was financially supported by Bulent Ecevit University Research Fund (Grant no. BEU-2016?33496813-01) and Kapadokya University #K?N(0).2018-BAGP-001. The authors thank Faruk Bahad?r for helping us to the preparation step of polymer.
Databáze: OpenAIRE
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