Bone marrow mononuclear cell therapy limits myocardial infarct size through vascular endothelial growth factor
| Autor: | Akira Takeshita, Qingwei Zhao, Kensuke Egashira, Shiro Kitamoto, Shin Nagata, Kenichi Hiasa, Minako Ishibashi, Masataka Sata, Shujiro Inoue, Weihua Ni, Makoto Katoh |
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| Rok vydání: | 2004 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Physiology Myocardial Infarction Infarction Apoptosis Mice SCID Pharmacology Peripheral blood mononuclear cell Cell therapy Mice chemistry.chemical_compound Physiology (medical) Internal medicine In Situ Nick-End Labeling Animals Medicine Myocardial infarction Bone Marrow Transplantation business.industry Myocardium medicine.disease Coronary Vessels Vascular endothelial growth factor Transplantation Disease Models Animal medicine.anatomical_structure chemistry Leukocytes Mononuclear Cardiology Bone marrow Cardiology and Cardiovascular Medicine Ligation business |
| Zdroj: | Basic Research in Cardiology. 99:165-172 |
| ISSN: | 1435-1803 0300-8428 |
| Popis: | No prior study has examined the effect of intravenous injection of bone marrow mononuclear cells (MNCs) on myocardial infarction size (IS). We tested the hypothesis that transplantation of MNCs decreases IS through the release of vascular endothelial growth factor (VEGF). Immediately after ligation of the left coronary artery of immunodeficient mice, PBS or MNCs were intravenously administered. Myocardial IS was significantly less in MNCs-treated mice than in PBS-treated mice. Trace experiments showed accumulation of exogenously administered MNCs into the vicinity of infarcted myocardium. Injection of MNCs did not affect capillary density after infarction, but did reduced myocardial cell apoptosis. Blockade of VEGF by a neutralizing antibody or by gene transfer of a soluble form of Flt-1 VEGF receptor diminished the IS-limiting effects of MNCs. In conclusion, injection of MNCs can reduce myocardial IS through the release of VEGF. The MNC therapy for acute myocardial infarction might improve prognosis of patients with myocardial infarction. |
| Databáze: | OpenAIRE |
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