Closed-channel culture system for efficient and reproducible differentiation of human pluripotent stem cells into islet cells
| Autor: | Alexander Turner, Shuhei Konagaya, Yuichiro Noda, Shigeru Kitamura, Hiroo Iwata, Hidetoshi Kotera, Kunio Hirano |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pluripotent Stem Cells Somatic cell Cellular differentiation Biophysics Enteroendocrine cell Nanotechnology Cell Separation Biology Biochemistry Regenerative medicine 03 medical and health sciences Islets of Langerhans Batch Cell Culture Techniques Lab-On-A-Chip Devices Humans Cellular Reprogramming Techniques Induced pluripotent stem cell Molecular Biology Cells Cultured Cell Differentiation Cell Biology Equipment Design Cell biology Transplantation Equipment Failure Analysis 030104 developmental biology |
| Zdroj: | Biochemical and biophysical research communications. 487(2) |
| ISSN: | 1090-2104 |
| Popis: | Human pluripotent stem cells (hPSCs) are thought to be a promising cell-source solution for regenerative medicine due to their indefinite proliferative potential and ability to differentiate to functional somatic cells. However, issues remain with regard to achieving reproducible differentiation of cells with the required functionality for realizing human transplantation therapies and with regard to reducing the potential for bacterial or fungal contamination. To meet these needs, we have developed a closed-channel culture device and corresponding control system. Uniformly-sized spheroidal hPSCs aggregates were formed inside wells within a closed-channel and maintained continuously throughout the culture process. Functional islet-like endocrine cell aggregates were reproducibly induced following a 30-day differentiation protocol. Our system shows an easily scalable, novel method for inducing PSC differentiation with both purity and functionality. |
| Databáze: | OpenAIRE |
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