Lipophilic chemicals from diesel exhaust particles trigger calcium response in human endothelial cells via aryl hydrocarbon receptor non-genomic signalling
| Autor: | Eric Le Ferrec, Bendik Christian Brinchmann, Klara Kukowski, Aubin Penna, Johan Øvrevik, Alena Kubátová, Dominique Lagadic-Gossmann, Jørn A. Holme, Kenji F. Shoji, Normand Podechard |
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| Přispěvatelé: | University of Oslo (UiO), Norwegian Institute of Public Health [Oslo] (NIPH), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), École des Hautes Études en Santé Publique [EHESP] (EHESP), University of North Dakota [Grand Forks] (UND), 228143, Norges Forskningsråd, Jonchère, Laurent, Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
genetic structures Calcium in biology lcsh:Chemistry 0302 clinical medicine Polycyclic Aromatic Hydrocarbons lcsh:QH301-705.5 diesel exhaust particle extracts endothelial cells calcium signalling membrane microdomains aryl hydrocarbon receptor Spectroscopy ComputingMilieux_MISCELLANEOUS Calcium signaling Vehicle Emissions Air Pollutants biology Chemistry General Medicine Store-operated calcium entry Computer Science Applications Membrane 030220 oncology & carcinogenesis Intracellular chemistry.chemical_element Calcium complex mixtures Catalysis Article Inorganic Chemistry 03 medical and health sciences Extracellular Humans [SDV.EE.SANT] Life Sciences [q-bio]/Ecology environment/Health Calcium Signaling Physical and Theoretical Chemistry Molecular Biology [SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/Health Organic Chemistry Aryl hydrocarbon receptor Atherosclerosis eye diseases 030104 developmental biology Receptors Aryl Hydrocarbon lcsh:Biology (General) lcsh:QD1-999 biology.protein Biophysics sense organs |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, 2018, 19 (5), pp.1429. ⟨10.3390/ijms19051429⟩ International Journal of Molecular Sciences; Volume 19; Issue 5; Pages: 1429 International Journal of Molecular Sciences, Vol 19, Iss 5, p 1429 (2018) International Journal of Molecular Sciences, MDPI, 2018, 19 (5), pp.1429. ⟨10.3390/ijms19051429⟩ |
| ISSN: | 1422-0067 1661-6596 |
| Popis: | Exposure to diesel exhaust particles (DEPs) affects endothelial function and may contribute to the development of atherosclerosis and vasomotor dysfunction. As intracellular calcium concentration [Ca2+]i is considered important in myoendothelial signalling, we explored the effects of extractable organic matter from DEPs (DEP-EOM) on [Ca2+]i and membrane microstructure in endothelial cells. DEP-EOM of increasing polarity was obtained by pressurized sequential extraction of DEPs with n-hexane (n-Hex-EOM), dichloromethane (DCM-EOM), methanol, and water. Chemical analysis revealed that the majority of organic matter was extracted by the n-Hex- and DCM-EOM, with polycyclic aromatic hydrocarbons primarily occurring in n-Hex-EOM. The concentration of calcium was measured in human microvascular endothelial cells (HMEC-1) using micro-spectrofluorometry. The lipophilic n-Hex-EOM and DCM-EOM, but not the more polar methanol- and water-soluble extracts, induced rapid [Ca2+]i increases in HMEC-1. n-Hex-EOM triggered [Ca2+]i increase from intracellular stores, followed by extracellular calcium influx consistent with store operated calcium entry (SOCE). By contrast, the less lipophilic DCM-EOM triggered [Ca2+]i increase via extracellular influx alone, resembling receptor operated calcium entry (ROCE). Both extracts increased [Ca2+]i via aryl hydrocarbon receptor (AhR) non-genomic signalling, verified by pharmacological inhibition and RNA-interference. Moreover, DCM-EOM appeared to induce an AhR-dependent reduction in the global plasma membrane order, as visualized by confocal fluorescence microscopy. DCM-EOM-triggered [Ca2+]i increase and membrane alterations were attenuated by the membrane stabilizing lipid cholesterol. In conclusion, lipophilic constituents of DEPs extracted by n-hexane and DCM seem to induce rapid AhR-dependent [Ca2+]i increase in HMEC-1 endothelial cells, possibly involving both ROCE and SOCE-mediated mechanisms. The semi-lipophilic fraction extracted by DCM also caused an AhR-dependent reduction in global membrane order, which appeared to be connected to the [Ca2+]i increase. |
| Databáze: | OpenAIRE |
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