Online chromatic and scale-space microvessel-tracing analysis for transmitted light optical images
| Autor: | Michael K. Griffiths, Simon Akerman, Meit A. Björndahl, Constantino Carlos Reyes-Aldasoro, Gillian M. Tozer, Jamila Ibrahim |
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| Rok vydání: | 2012 |
| Předmět: |
Optics and Photonics
Pathology medicine.medical_specialty Time Factors Light media_common.quotation_subject Transmitted light Color Tracing Biochemistry Phosphates Scale space Mice Cell Line Tumor Bibenzyls medicine Animals Humans Contrast (vision) Chromatic scale Microvessel media_common Internet Models Statistical business.industry Microcirculation Cell Biology Oxygen TA Vascular morphology Microvessels Cardiology and Cardiovascular Medicine business Algorithms Intravital microscopy Biomedical engineering |
| Zdroj: | Microvascular Research |
| ISSN: | 0026-2862 |
| Popis: | Limited contrast in transmitted light optical images from intravital microscopy is problematic for analysing tumour vascular morphology. Moreover, in some cases, changes in vasculature are visible to a human observer but are not easy to quantify. In this paper two online algorithms are presented: scale-space vessel tracing and chromatic decomposition for analysis of the vasculature of SW1222 human colorectal carcinoma xenografts growing in dorsal skin-fold "window" chambers in mice. Transmitted light optical images of tumours were obtained from mice treated with the tumour vascular disrupting agent, combretastatin-A-4-phosphate (CA4P), or saline. The tracing algorithm was validated against hand-traced vessels with accurate results. The measurements extracted with the algorithms confirmed the known effects of CA4P on tumour vascular topology. Furthermore, changes in the chromaticity suggest a deoxygenation of the blood with a recovery to initial levels in CA4P-treated tumours relative to the controls. The algorithms can be freely applied to other studies through the CAIMAN website (CAncer IMage ANalysis: http://www.caiman.org.uk). |
| Databáze: | OpenAIRE |
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