Role of the adenylate cyclase system in altered insulin release from islets of Langerhans of aging rats
| Autor: | Loren G. Lipson, Gretchen E. Tietjen, Michael J. Bush, Aeree Yoon |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Blood Glucose
Male Aging endocrine system medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Adenylate kinase Deoxyglucose Cyclase Islets of Langerhans Endocrinology Internal medicine Insulin Secretion medicine Animals Insulin geography geography.geographical_feature_category Phosphoric Diester Hydrolases Chemistry Proteins Phosphodiesterase General Medicine Islet In vitro Rats Hyperglycemia Collagenase Cyclase activity Adenylyl Cyclases medicine.drug |
| Zdroj: | Acta Endocrinologica. 96:222-226 |
| ISSN: | 1479-683X 0804-4643 |
| DOI: | 10.1530/acta.0.0960222 |
| Popis: | In attempting to understand the causes of the hyperglycaemia observed in aging populations and to determine the mechanism(s) for the diminished in vitro insulin release from islets of Langerhans of older rats, the adenylate cyclase-cyclic AMP system was studied in isolated islets from 12 month old and 2½ month old (control) male rats to determine its role in this altered insulin secretion. Islets of Langerhans were isolated by collagenase digestion and then either incubated in the presence of low or high concentrations of glucose for studies of insulin release or were sonicated and assayed for determinations of activities of adenylate cyclase and phosphodiesterase. Insulin release was identical from islets of 12 month old and 2½ month old rats to 2.8 mm D-glucose, while in the presence of 16.7 mm D-glucose, insulin release was decreased by 33% (P < 0.02) from islets of the older animals. Adenylate cyclase activity was diminished by 60% (P < 0.005) from the 12 month old rats as compared with islets from the 2½ month old controls, while low Km phosphodiesterase activity was similar in islets from both groups of animals. From these studies it appears that the adenylate cyclase-cyclic AMP system may play a role in the altered insulin release from islets of aging rats. |
| Databáze: | OpenAIRE |
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