Treatment with crocin improves cardiac dysfunction by normalizing autophagy and inhibiting apoptosis in STZ-induced diabetic cardiomyopathy
Autor: | Kyriakos Mellidis, Eleftheria Galatou, Z. Sinakos, Konstantinos Feidantsis, Antigone Lazou |
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Rok vydání: | 2018 |
Předmět: |
Blood Glucose
Male 0301 basic medicine Cardiac function curve Diabetic Cardiomyopathies Endocrinology Diabetes and Metabolism Autophagy-Related Proteins Medicine (miscellaneous) Apoptosis Pharmacology Streptozocin Ventricular Function Left Diabetes Mellitus Experimental Crocin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Fibrosis Diabetes mellitus Diabetic cardiomyopathy Autophagy medicine Animals HSP70 Heat-Shock Proteins Myocytes Cardiac Rats Wistar Nutrition and Dietetics Ventricular Remodeling business.industry AMPK Recovery of Function medicine.disease Streptozotocin Carotenoids 030104 developmental biology chemistry 030220 oncology & carcinogenesis Apoptosis Regulatory Proteins Cardiology and Cardiovascular Medicine business Signal Transduction medicine.drug |
Zdroj: | Nutrition, Metabolism and Cardiovascular Diseases. 28:952-961 |
ISSN: | 0939-4753 |
Popis: | Background and aim The association of diabetes mellitus (DM) and poor metabolic control with high incidence of cardiovascular diseases is well established. The aim of this study was to investigate the potential cardioprotective effect of crocin (Crocus sativus L. extract) on diabetic heart dysfunction and to elucidate the mediating molecular mechanisms. Methods and results Streptozotocin (STZ)-induced diabetic rats were treated with two different concentrations of crocin (10 or 20 mg/kg), while isolated cardiac myocytes exposed to 25 mM glucose, were treated with 1 or 10 μM of crocin. Treatment of STZ-diabetic rats with crocin resulted in normalization of plasma glucose levels, inhibition of cardiac hypertrophy and fibrosis, and improvement of cardiac contractile function. Heat Shock Response was enhanced. Myocardial AMPK phosphorylation was increased after treatment with crocin, resulting in normalization of autophagy marker proteins (LC3BII/LC3BI ratio, SQSTM1/p62 and Beclin-1), while the diabetes-induced myocardial apoptosis was decreased. Similar results regarding the effect of crocin on autophagy and apoptosis pathways were obtained in isolated cardiac myocytes exposed to high concentration of glucose. Conclusion The results suggest that crocin improves the deteriorated cardiac function in diabetic animals by enhancing the heat shock response, inhibiting apoptosis and normalizing autophagy in cardiac myocytes. Thus, treatment with crocin may represent a novel approach for treating diabetic cardiomyopathy. |
Databáze: | OpenAIRE |
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