Treatment with crocin improves cardiac dysfunction by normalizing autophagy and inhibiting apoptosis in STZ-induced diabetic cardiomyopathy

Autor: Kyriakos Mellidis, Eleftheria Galatou, Z. Sinakos, Konstantinos Feidantsis, Antigone Lazou
Rok vydání: 2018
Předmět:
Blood Glucose
Male
0301 basic medicine
Cardiac function curve
Diabetic Cardiomyopathies
Endocrinology
Diabetes and Metabolism
Autophagy-Related Proteins
Medicine (miscellaneous)
Apoptosis
Pharmacology
Streptozocin
Ventricular Function
Left
Diabetes Mellitus
Experimental
Crocin
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Fibrosis
Diabetes mellitus
Diabetic cardiomyopathy
Autophagy
medicine
Animals
HSP70 Heat-Shock Proteins
Myocytes
Cardiac
Rats
Wistar
Nutrition and Dietetics
Ventricular Remodeling
business.industry
AMPK
Recovery of Function
medicine.disease
Streptozotocin
Carotenoids
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Apoptosis Regulatory Proteins
Cardiology and Cardiovascular Medicine
business
Signal Transduction
medicine.drug
Zdroj: Nutrition, Metabolism and Cardiovascular Diseases. 28:952-961
ISSN: 0939-4753
Popis: Background and aim The association of diabetes mellitus (DM) and poor metabolic control with high incidence of cardiovascular diseases is well established. The aim of this study was to investigate the potential cardioprotective effect of crocin (Crocus sativus L. extract) on diabetic heart dysfunction and to elucidate the mediating molecular mechanisms. Methods and results Streptozotocin (STZ)-induced diabetic rats were treated with two different concentrations of crocin (10 or 20 mg/kg), while isolated cardiac myocytes exposed to 25 mM glucose, were treated with 1 or 10 μM of crocin. Treatment of STZ-diabetic rats with crocin resulted in normalization of plasma glucose levels, inhibition of cardiac hypertrophy and fibrosis, and improvement of cardiac contractile function. Heat Shock Response was enhanced. Myocardial AMPK phosphorylation was increased after treatment with crocin, resulting in normalization of autophagy marker proteins (LC3BII/LC3BI ratio, SQSTM1/p62 and Beclin-1), while the diabetes-induced myocardial apoptosis was decreased. Similar results regarding the effect of crocin on autophagy and apoptosis pathways were obtained in isolated cardiac myocytes exposed to high concentration of glucose. Conclusion The results suggest that crocin improves the deteriorated cardiac function in diabetic animals by enhancing the heat shock response, inhibiting apoptosis and normalizing autophagy in cardiac myocytes. Thus, treatment with crocin may represent a novel approach for treating diabetic cardiomyopathy.
Databáze: OpenAIRE
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