Untargeted Metabolomics Reveals Lipid Impairment in the Liver of Adult Zebrafish ( Danio rerio ) Exposed to Carbendazim

Autor: Raíssa M. Costa, Victória R. Matos e Chaib, Anderson G. Domingues, Karina T. S. Rubio, Maria Elvira Poleti Martucci
Rok vydání: 2023
Předmět:
Zdroj: Environmental Toxicology and Chemistry. 42:437-448
ISSN: 1552-8618
0730-7268
DOI: 10.1002/etc.5534
Popis: Carbendazim is a systemic fungicide used in several countries, particularly in Brazil. However, studies suggest that it is related to the promotion of tumors, endocrine disruption, toxicity to organisms, among other effects. As a result, carbendazim is not allowed in the USA, Australia and in some European Union countries. Therefore, further studies are necessary to evaluate its effects and zebrafish is a model routinely used to provide relevant information regarding the acute and long-term effects of xenobiotics. In this way, zebrafish water tank samples (water samples of aquarium containing zebrafish) and liver samples from animals exposed to carbendazim at a concentration of 120 ug/L were analyzed by liquid chromatography coupled to high-resolution mass spectrometry (LC- HRMS), followed by multivariate and univariate statistical analyses, using the metabolomics approach. Our results suggest impairment of lipid metabolism with a consequent increase in intrahepatic lipids and endocrine disruption. Furthermore, the results suggest two endogenous metabolites as potential biomarkers to determine carbendazim exposure. Finally, this study showed to be possible using zebrafish water tank to assess the dysregulation of endogenous metabolites followed by an understanding of the biological effects. This article is protected by copyright. All rights reserved. Environ Toxicol Chem 2022;00:0-0. © 2022 SETAC.
Databáze: OpenAIRE
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