Analysis of in vitro transcription products of intracellular vesicular stomatitis virus RNA polymerase
| Autor: | D Hallett, Helene Galet, Ludvik Prevec |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Transcription
Genetic Immunology RNA-dependent RNA polymerase Biology Microbiology Defective virus Vesicular stomatitis Indiana virus Vesicular Stomatitis chemistry.chemical_compound L Cells Transcription (biology) Virology RNA polymerase Transcriptional regulation Base Sequence Cell-Free System Temperature RNA Defective Viruses DNA-Directed RNA Polymerases biology.organism_classification Molecular biology chemistry Vesicular stomatitis virus Insect Science RNA Viral Research Article |
| Zdroj: | Journal of virology. 19(2) |
| ISSN: | 0022-538X |
| Popis: | The intracellular transcriptase complex of vesicular stomatitis virus-infected L cells synthesized RNA complementary to the entire infectious virus genome at either 37 degrees C or 28 degrees C in vitro. Not all sequences were present at the same frequency, however; copies of that segment of the genome common to the LT defective particles were present at 20 to 100 times higher frequently than copies of the genome segment common to the ST defective particle. The less frequent region was transcribed somewhat more effectively at 28 degrees C than at 37 degrees C. The results suggest that transcriptional regulation rather than selective degradation is responsible for the differential accumulation of RNA. |
| Databáze: | OpenAIRE |
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