Ex-vivo fluorescence confocal microscopy with digital staining for characterizing basal cell carcinoma on frozen sections: A comparison with histology
| Autor: | Cristel Ruini, Gabriela Vladimirova, Daniela Hartmann, Ecem Ergun, Benjamin Kendziora, Suzanna Salzer, Lars E. French, Elke Sattler |
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| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty Skin Neoplasms Basal Cell H&E stain General Physics and Astronomy 01 natural sciences General Biochemistry Genetics and Molecular Biology law.invention micrographic surgery 010309 optics ex-vivo confocal microscopy chemistry.chemical_compound basal cell carcinoma Confocal microscopy law Fresh Tissue 0103 physical sciences medicine Frozen Sections Humans General Materials Science Basal cell carcinoma Frozen section procedure Microscopy Microscopy Confocal Staining and Labeling Chemistry 010401 analytical chemistry Acridine orange Carcinoma General Engineering Histology General Chemistry bedside histology medicine.disease Mohs Surgery 0104 chemical sciences Staining Carcinoma Basal Cell Confocal digital staining |
| Zdroj: | Journal of biophotonicsREFERENCES. 14(8) |
| ISSN: | 1864-0648 |
| Popis: | Ex-vivo fluorescence confocal microscopy (FCM) has been used on fresh tissue, but there is little experience on frozen sections. We evaluated the applicability of FCM on frozen sections of basal cell carcinomas (BCCs), stained with acridine orange and digitally colored to simulate hematoxylin and eosin (H&E) dyes. We compared our diagnostic accuracy in detecting and subtyping BCCs with FCM to our gold standard (H&E stained frozen sections used in 3D horizontal micrographic surgery). Fourty-six primary BCCs were analyzed for free margins as well as histological subtype with all FCM modes and conventional H&E staining. Adnexa, artifacts and diagnostic confidence were evaluated. Free margins were identified with a sensitivity and specificity of 92% and 91%. Concordance for tumor subtype was 88%. FCM may be used on both fresh tissue and frozen samples, although with reduced performance and different artifacts. The device is useful for the intraoperative diagnosis, subtyping and margin-mapping of BCCs. |
| Databáze: | OpenAIRE |
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