Microcontact printing of proteins on oxygen plasma-activated poly(methyl methacrylate)
| Autor: | Kristine Schmalenberg, Kathryn E. Uhrich, Helen M. Buettner |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Hot Temperature
Materials science Surface Properties Cell Culture Techniques Biophysics Schwann cell Bioengineering Chick Embryo macromolecular substances Biomaterials chemistry.chemical_compound Coated Materials Biocompatible Laminin Materials Testing Polymer chemistry Cell Adhesion medicine Animals Polymethyl Methacrylate Methyl methacrylate Cells Cultured Neurons chemistry.chemical_classification biology Microchemistry technology industry and agriculture Cell Polarity Polymer Poly(methyl methacrylate) Rats Oxygen medicine.anatomical_structure nervous system chemistry Mechanics of Materials visual_art Microcontact printing Ceramics and Composites visual_art.visual_art_medium biology.protein Printing Adsorption Schwann Cells Cell Division Protein adsorption Micropatterning |
| Zdroj: | Biomaterials. 25:1851-1857 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2003.08.048 |
| Popis: | This paper describes a method for microcontact printing protein solutions onto polymer substrates temporarily activated by oxygen plasma. Following plasma treatment, poly(dimethyl siloxane) (PDMS) stamps were coated with an aqueous laminin solution then placed in direct contact with plasma-treated poly(methyl methacrylate) (PMMA) substrates. This process resulted in well defined laminin stripes on the PMMA surface when printing was performed within 45min of the plasma treatment. Axonal outgrowth from embryonic chick dorsal root ganglia (DRG) was largely confined to the stamped pattern, while over 90% of primary rat Schwann cells adhered to the protein stamped areas on the PMMA substrates. Oxygen-plasma treatment of the PMMA surface was necessary to deposit proteins that direct axonal outgrowth from chick DRG and Schwann cell adherence. |
| Databáze: | OpenAIRE |
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