Production of benzyl cinnamate by a low-cost immobilized lipase and evaluation of its antioxidant activity and toxicity
| Autor: | Rogério Luis Cansian, Suelen P. Piazza, Rogério Marcos Dallago, Natalia Paroul, Débora de Oliveira, Bruna Maria Saorin Puton, Marcelo Luis Mignoni |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Optimization
Antioxidant biology DPPH Operational cycle lcsh:Biotechnology medicine.medical_treatment biology.organism_classification Applied Microbiology and Biotechnology Acute toxicity Cinnamic acid Enzyme catalysis chemistry.chemical_compound chemistry lcsh:TP248.13-248.65 Lipase NS 8801 medicine Benzyl cinnamate Artemia salina Enzymatic esterification IC50 Biotechnology Nuclear chemistry ComputingMethodologies_COMPUTERGRAPHICS Research Article |
| Zdroj: | Biotechnology Reports Biotechnology Reports, Vol 29, Iss, Pp e00586-(2021) |
| ISSN: | 2215-017X |
| Popis: | Graphical abstract Highlights • Benzyl cinnamate production was optimized by enzymatic esterification. • The lipase NS 8011 showed efficiency and stability until the 13th cycle. • Benzyl cinnamate showed antioxidant activity and toxicity against Artemia salina. In this work was optimized the production of benzyl cinnamate by enzymatic catalysis using the immobilized lipase NS88011 and to evaluate its biological properties. The optimized condition for this system was 1:3 (acid:alcohol) molar ratio, 59 °C, biocatalyst concentration 4.4 mg.mL−1 for 32 h, with a yield of 97.6 %. The enzyme stability study showed that the enzyme remains active and yields above 60 % until the 13th cycle (416 h), presenting a promising half-life. In the determination of the antioxidant activity of the ester, an inhibitory concentration necessary to inhibit 50 % of the free radical 2,2-diphenyl-1-picryl-hydrazyl DPPH (IC50) of 149.8 mg.mL−1 was observed. For acute toxicity against bioindicator Artemia salina, lethal doses (LD50) of 0.07 and 436.7 μg.mL−1 were obtained for the ester and cinnamic acid, showing that benzyl cinnamate had higher toxicity, indicating potential cytotoxic activity against human tumors. |
| Databáze: | OpenAIRE |
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