Increasing Incidence of Colon Cancer in the Young: Assessing the Tumor Biology
Autor: | Jana Jandova, Pamela Omesiete, Agnes Ewongwo, Aaron Scott, Emad Elquza, Hunter Jecius, Debbie Chen, Valentine Nfonsam |
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Rok vydání: | 2018 |
Předmět: |
Male
Colorectal cancer Enzyme-Linked Immunosorbent Assay Cartilage Oligomeric Matrix Protein medicine.disease_cause 03 medical and health sciences 0302 clinical medicine Carcinoembryonic antigen Blood serum Biomarkers Tumor Medicine Humans Propensity Score Neoplasm Staging Cartilage oligomeric matrix protein biology business.industry Incidence Age Factors Arizona Cancer DNA Neoplasm Middle Aged medicine.disease Gene Expression Regulation Neoplastic Vascular endothelial growth factor C 030220 oncology & carcinogenesis Colonic Neoplasms biology.protein Cancer research Biomarker (medicine) 030211 gastroenterology & hepatology Surgery Female business Carcinogenesis |
Zdroj: | Journal of the American College of Surgeons. 229(1) |
ISSN: | 1879-1190 |
Popis: | The overall incidence of colon cancer (CC) is decreasing, but with increasing early-onset colon cancer (EOCC50 years old). Our recent study revealed unique overexpression of cartilage oligomeric matrix protein (COMP) in EOCC and its association with aggressiveness. The aim of this study was to assess CC biology, especially in the young, by evaluating the role of COMP in CC carcinogenesis and cancer progression, detecting COMP in serum and its association with disease stage.Cancer and matching noninvolved tissue blocks from 12 sporadic EOCC and late-onset colon cancer (LOCC) patients of 4 disease stages were obtained from pathology archives. Ribonucleic acid expression profiling of 770 cancer-related genes using nCounter platform was performed. The COMP levels from 16 EOCC and LOCC serum samples were measured by ELISA. Carcinoembryonic antigen levels from these 16 samples were taken at the time of diagnosis. Transwell assay was performed to elucidate the role of COMP in motility and metastases.Expression profiling revealed increased COMP levels in higher disease stage. There was 7-fold higher COMP expression (p ≤ 0.05) in stage III compare to stage I and its coexpression with GAS1, VEGFC, MAP3K8, SFRP1, and PRKACA. Higher COMP expression was seen in stage II compared with stage I (p = 0.07) and its coexpression withTLR2, IL8, RIN1, IRAK3, and CACNA2D2, and COMP was detectable in serum and showed significantly higher levels in EOCC compared with LOCC. Similar correlation was seen with CEA levels, but the difference was not significant. Transwell assay revealed significantly increased motility of HT-29 cells after treatment with recombinant COMP.These findings suggest different tumor biology between EOCC and LOCC. Cartilage oligomeric matrix protein plays a significant role in CC carcinogenesis and has potential as biomarker for CC, especially aggressive EOCC. |
Databáze: | OpenAIRE |
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