A comparative analysis of phenotype expression in human osteoblasts from heterotopic ossification and normal bone
| Autor: | Otmar Trentz, Sonja Hemmi, Volker Wedler, Omana A. Trentz, Alexander E. Handschin, Marcus Egermann |
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| Přispěvatelé: | University of Zurich, Handschin, Alexander E |
| Rok vydání: | 2006 |
| Předmět: |
Adult
Genetic Markers Male musculoskeletal diseases Pathology medicine.medical_specialty Osteocalcin Gene Expression 610 Medicine & health Core Binding Factor Alpha 1 Subunit 142-005 142-005 Bone and Bones Collagen Type I Fracture Fixation Internal Gene expression Humans Medicine RNA Messenger Cells Cultured Bone Transplantation Osteoblasts biology Reverse Transcriptase Polymerase Chain Reaction business.industry Ossification Heterotopic Cell Differentiation Osteoblast Middle Aged Alkaline Phosphatase medicine.disease Phenotype Molecular biology 2746 Surgery Reverse transcription polymerase chain reaction medicine.anatomical_structure biology.protein Alkaline phosphatase Female Surgery Heterotopic ossification business Type I collagen |
| Zdroj: | Handschin, Alexander E; Egermann, Marcus; Wedler, Volker; Trentz, Otmar; Hemmi, Sonja; Trentz, Omana A (2006). A comparative analysis of phenotype expression in human osteoblasts from heterotopic ossification and normal bone. Langenbeck's archives of surgery, 391(4), pp. 376-82. Berlin: Springer 10.1007/s00423-005-0021-5 |
| DOI: | 10.48350/21740 |
| Popis: | BACKGROUND AND AIMS: Heterotopic ossification (HO) is a pathological bone formation process in which ectopic bone is formed in soft tissue. The formation of bone depends on the expression of the osteoblast phenotype. Earlier studies have shown conflicting results on the expression of phenotype markers of cells originating from HO and normal bone. The hypothesis of the present study is that cells from HO show an altered expression of osteoblast-specific phenotype markers compared to normal osteoblasts. The aims of the study were to further characterize the expression of osteoblast phenotypemarkers and to provide a comparison with other study results. PATIENTS AND METHODS: Using an in vitro technique, reverse transcription polymerase chain reaction (RT-PCR), real-time PCR and immunohistochemistry, we compared the phenotype gene expression (type I collagen, alkaline phosphatase, Cbfa-1, osteocalcin) of osteoblasts from resected HO and normal bone (iliac crest). RESULTS: Cells from HO expressed the osteoblast phenotype (type I collagen, alkaline phosphatase) but were characterized by a depleted osteocalcin expression. The expression of Cbfa-1 (osteocalcin transcription gene) showed a large variety in our study. Preoperative radiotherapy had no effect on phenotype expression in cells from HO. CONCLUSION: Our results provide a characterization of cells originating from HO and support the thesis of an impaired osteoblast differentiation underlying the formation of HO. The transcription axis from Cbfa-1 to osteocalcin could be involved in the pathogenesis of HO. |
| Databáze: | OpenAIRE |
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