Pulsed field gel electrophoresis as a new epidemiological tool for monitoring methicillin-resistant Staphylococcus aureus in an intensive care unit
| Autor: | M. Bientz, J. M. Mantz, Y. Piemont, G. Prevost, B. Pottecher, M. Dahlet |
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| Rok vydání: | 1991 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Staphylococcus aureus Micrococcaceae medicine.disease_cause law.invention Microbiology Plasmid law medicine Pulsed-field gel electrophoresis Humans Typing Bacteriophage Typing Electrophoresis Agar Gel biology business.industry Reproducibility of Results General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Methicillin-resistant Staphylococcus aureus Intensive care unit Intensive Care Units Infectious Diseases Carriage Methicillin Resistance business Plasmids |
| Zdroj: | Journal of Hospital Infection. 17:255-269 |
| ISSN: | 0195-6701 |
| DOI: | 10.1016/0195-6701(91)90270-i |
| Popis: | Pulsed field gel electrophoresis (PFGE) of bacterial DNA was used in a 1-month epidemiological study of methicillin-resistant Staphylococcus aureus (MRSA) in a 15-bed Intensive Care Unit (ICU). Patient and hospital staff carriage as well as distribution of MRSA in the ICU environment were investigated, and a total of 3802 samples produced 175 isolates. The stability and the reproducibility of the PFGE method were satisfactory. Moreover, the plasmid content of the strains so far examined had no influence on the PFGE profiles of the MRSA strains. The polymorphic profiles observed also account for the use of this method as an epidemiological tool for investigating MRSA. Among 30 patients who stayed more than 4 days in the unit, PFGE analysis showed 11 episodes of colonization in nine patients, whereas lysotyping and plasmid DNA analysis demonstrated only eight and seven such episodes in the same patients, respectively. The combination of PFGE with lysotyping and plasmid analysis may provide a greater discriminatory capacity between MRSA isolates. |
| Databáze: | OpenAIRE |
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