Quantitation of artemisinin and its biosynthetic precursors in Artemisia annua L. by high performance liquid chromatography–electrospray quadrupole time-of-flight tandem mass spectrometry
| Autor: | Jan Van Bocxlaer, Filip Van Nieuwerburgh, Lies Maes, Dieter Deforce, Sofie Vande Casteele, Alain Goossens, Dirk Inzé |
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| Rok vydání: | 2006 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Electrospray Chromatography biology Chemistry Organic Chemistry Artemisia annua Reproducibility of Results Ion suppression in liquid chromatography–mass spectrometry General Medicine Reversed-phase chromatography Reference Standards Tandem mass spectrometry Mass spectrometry biology.organism_classification Sensitivity and Specificity Biochemistry High-performance liquid chromatography Artemisinins Analytical Chemistry Standard curve Artemisia Sesquiterpenes Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A. 1118:180-187 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2006.03.121 |
| Popis: | This study reports the development and validation of a rapid, sensitive and selective assay for the quantitation of artemisinin, arteannuin B, artemisitene and artemisinic acid in Artemisia annua L. by reversed phase high performance liquid chromatography (HPLC) electrospray (ESI) quadrupole time of flight (Q-TOF) tandem mass spectrometry (MS/MS). A recovery of >97% for all analytes was achieved by immersing one gram of fresh plant material in chloroform for 1 min. This result supports the hypothesis that artemisinin and some of its structural analogs present in the leaves A. annua L. are localized entirely in the subcuticular space of the glands on the surface of the leaves. We validated the use of this chloroform extract, without additional sample preparation steps, for quantitative Q-TOF MS/MS. No ion suppression (matrix effect) resulting from interference with other compounds was detected. For every concentration within the range of the standard curve (0.1 to 3.00 microg/ml), accuracy was between 85% and 115%. Within- and between-day variations for the analysis of A. annua L. samples were |
| Databáze: | OpenAIRE |
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