In Vitro antioxidant and antibacterial activity of leaf extracts of Measa lanceolata

Autor:	Workineh Mengesha Fereja, Engeda Dessalegn, Jundi Ismael
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	chemistry.chemical_classification Antioxidant antioxidant measa lanceolata Nutrition. Foods and food supply medicine.medical_treatment fungi Flavonoid food and beverages phenolic compounds TP368-456 In vitro Food processing and manufacture antibacterial chemistry medicine TX341-641 Food science Antibacterial activity Food Science
Zdroj:	International Journal of Food Properties, Vol 24, Iss 1, Pp 702-712 (2021)
ISSN:	1532-2386 1094-2912
Popis:	This study investigated total phenolic contents (TPC), total flavonoid contents (TFC), antioxidant activity, and antibacterial activities of different leaf extracts of Measa lanceolata. The TPC and TFC of the extracts were determined by Folin-Ciocalteu and aluminum chloride methods, respectively. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, reducing power, and phosphomolybedinium assays were used to evaluate antioxidant activity. Antibacterial properties were assessed using the disc-diffusion assay based on minimum inhibitory concentration (MIC). Bacteria tested were three strains of gram-negative (Escherichia coli (ATCC-25922), Proteus vulgaris (ATCC-13315), and Pseudomonas aeruginosa (ATCC-43495)), and two strains of gram-positive (Staphylococcus aureus(ATCC-25923), and Enterococcus faecalis(ATCC-29212)). It was found that methanol extract contained the highest TPC (60.6 ± 4.4 mg of gallic acid equivalent/g of dried extract) and TFC (6.4 ± 6.0 mg of catechin equivalent/g of dried extract). Extract showed the strongest DPPH radical scavenging activity (EC50 = 76.7 ± 7.3 µg/mL), iron reducing power (EC50 = 74.0 ± 1.6 µg/mL), and total antioxidant activity (128 ± 4 mg of ascorbic acid equivalent per dried extract). Inhibition zones were observed in water and methanol extracts against bacterial strains, whereas Staphylococcus aureus (ATCC-25923), Escherichia coli(ATCC-25922), Pseudomonas aeruginosa (ATCC-43495), and Proteus vulgaris(ATCC-13315) were resistant against chloroform and ethyl-acetate extracts.
Databáze:	OpenAIRE
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