Preservation of collagen in the soft tissues of frozen mammoths
| Autor: | Mitsutaka Miura, Testuya Ebihara, Shunji Hattori, Tomomi Kiriyama-Tanaka, Haruki Senoo, Alexei Tikhonov, Masashi Kusubata, Yuki Taga, Yuki Kumazawa, Yoshihiro Mezaki, Katsuyuki Imai |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Physiology
Elephants Permafrost Biochemistry Mass Spectrometry Diagnostic Radiology Extracellular matrix Mammoths Amino acid analysis Sequence Analysis Protein Medicine and Health Sciences Lung Gel Electrophoresis Mammals Staining Multidisciplinary biology Fossils Chemistry Radiology and Imaging Eukaryota Soft tissue Trypsin Pulmonary Imaging Extracellular Matrix Liver Physiological Parameters Vertebrates Amino Acid Analysis Medicine Immunohistochemistry Female Collagen Anatomy Digestion Research Article medicine.drug Collagen Type IV Silver Staining Soft Tissues Imaging Techniques Science Preservation Biological Electrophoretic Staining Research and Analysis Methods Collagen Type I Electrophoretic Techniques Diagnostic Medicine medicine Animals Molecular Biology Techniques Molecular Biology Mammoth Molecular Biology Assays and Analysis Techniques Body Weight Organisms Biology and Life Sciences Proteins Kidneys Renal System biology.organism_classification Molecular biology Siberia Biological Tissue Specimen Preparation and Treatment Amniotes Ultrastructure Collagens Zoology Chromatography Liquid |
| Zdroj: | PLoS ONE, Vol 16, Iss 10, p e0258699 (2021) PLoS ONE PLoS ONE, Vol 16, Iss 10 (2021) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0258699 |
| Popis: | We investigated the characteristics of extracellular matrix (ECM) in the soft tissue of two frozen baby woolly mammoths (Mammuthus primigenius) that died and were buried in Siberian permafrost approximately 40,000 years ago. Morphological and biochemical analyses of mammoth lung and liver demonstrated that those soft tissues were preserved at the gross anatomical and histological levels. The ultrastructure of ECM components, namely a fibrillar structure with a collagen-characteristic pattern of cross-striation, was clearly visible with transmission and scanning electron microscopy. Type I and type IV collagens were detected by immunohistochemical observation. Quantitative amino acid analysis of liver and lung tissues of the baby mammoths indicated that collagenous protein is selectively preserved in these tissues as a main protein. Type I and type III collagens were detected as major components by means of liquid chromatography–mass spectrometry analysis after digestion with trypsin. These results indicate that the triple helical collagen molecule, which is resistant to proteinase digestion, has been preserved in the soft tissues of these frozen mammoths for 40,000 years. |
| Databáze: | OpenAIRE |
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