Determination of methionine-enkephalin and leucine-enkephalin by LC-MS in human plasma: Study of pre-analytical stability
| Autor: | Aliye Ozalp, John Meijer, Cas van den Beld, Begona Barroso |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Detection limit Reproducibility Chromatography Molecular Structure Chemistry Enkephalin Methionine Biophysics Cell Biology Biochemistry High-performance liquid chromatography Mass Spectrometry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Liquid chromatography–mass spectrometry Protein precipitation Humans Sample collection Opioid peptide Molecular Biology Quantitative analysis (chemistry) 030217 neurology & neurosurgery Chromatography High Pressure Liquid Enkephalin Leucine |
| Zdroj: | Analytical biochemistry. 559 |
| ISSN: | 1096-0309 |
| Popis: | The aim of this work was to assess the influence of preanalytical variables on the stability of two endogenous opioid peptides (Methionine-Enkephalin and Leucine-Enkephalin) in human plasma. For this purpose, first a sensitive LC-MS/MS analytical method was developed and validated for the simultaneous quantitative analysis of these two peptides. The methodology consisted of a simple protein precipitation step followed by UPLC separation and MRM quantitative analysis using a stable isotope labelled Methionine-Enkephalin as internal standard. The method with a limit of quantitation of 10 pg/mL showed good reproducibility with excellent accuracy and precision, and was linear up to 2000 pg/mL. An extensive evaluation of the pre-analytical stability of these peptides in human blood was carried out to ensure an adequate sample collection procedure to obtain reliable results in the analysis of clinical samples. |
| Databáze: | OpenAIRE |
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