Establishment of an anti-inflammation-based bioassay for the quality control of the 13-component TCM formula (Lianhua Qingwen)

Autor: Ming Niu, Jiabo Wang, Xiaojuan Yang, Yanru Zhang, Xiaohe Xiao, Zi-Teng Zhang, Zhuo Shi, Ziying Wei, Haizhu Zhang, Jianli Ma, Shuai-Shuai Chen, Ying Huang
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Pharmaceutical Biology, Vol 59, Iss 1, Pp 537-545 (2021)
Pharmaceutical Biology
article-version (VoR) Version of Record
ISSN: 1744-5116
1388-0209
Popis: Context Owing to the complexity of chemical ingredients in traditional Chinese medicine (TCM), it is difficult to maintain quality and efficacy by relying only on chemical markers. Objective Lianhua Qingwen capsule (LHQW) was selected as an example to discuss the feasibility of a bioassay for quality control. Materials and methods Network pharmacology was used to screen potential targets in LHQW with respect to its anti-inflammatory effects. An in vitro cell model was used to validate the prediction. An anti-inflammatory bioassay was established for the quality evaluation of LHQW in 40 batches of marketed products and three batches of destructed samples. Results The tumor necrosis factor/interleukin-6 (TNF/IL-6) pathway via macrophage was selected as the potential target of LHQW. The IC50 value of LHQW on RAW 264.7 was 799.8 μg/mL. LHQW had significant inhibitory effects on the expression of IL-6 in a dose-dependent manner (p
Databáze: OpenAIRE
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