Expression of recombinant proteins of Orientia Tsutsugamushi and their applications in the serodiagnosis of scrub typhus
| Autor: | Shamala Devi, Ho Tm, M. Y. Rohani, Sun Tee Tay |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Orientia tsutsugamushi Molecular Sequence Data Enzyme-Linked Immunosorbent Assay Scrub typhus Cross Reactions Polymerase Chain Reaction Sensitivity and Specificity Microbiology law.invention Serology Immunoenzyme Techniques Species Specificity Antigen law Rickettsia typhi medicine Animals Humans Serologic Tests Base Sequence biology General Medicine bacterial infections and mycoses medicine.disease biology.organism_classification Antibodies Bacterial Virology Recombinant Proteins Infectious Diseases Rickettsiosis Scrub Typhus biology.protein Recombinant DNA bacteria Rabbits Antibody |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 44:137-142 |
| ISSN: | 0732-8893 |
| DOI: | 10.1016/s0732-8893(02)00434-0 |
| Popis: | In this study, recombinant proteins that encompassed the AD I-AD III regions of 56 kDa immunodominant gene of 2 Orientia tsutsugamushi (OT) serotypes; Gilliam and TA763 were expressed in Escherichia coli. Both recombinant proteins exhibited serologic cross-reactivity with the rabbit antisera against various OT serotypes, as evaluated by enzyme-linked immunosorbent assay (ELISA), but not against other rickettsial species, including Rickettsia typhi, R. prowazekii and TT118 SFG rickettsiae. The feasibility of using the recombinant proteins as a diagnostic reagent was further evaluated by ELISA using sera from blood donors and scrub typhus patients. The results suggested a higher affinity of the antihuman IgM than IgG with both recombinant proteins. The IgM ELISA findings were agreeable with the results of indirect immunoperoxidase (IIP) assay especially with sera of high antibody (1:1,600). However, more than one antigen are probably needed for development of an effective assay for serodiagnosis of scrub typhus in endemic areas. |
| Databáze: | OpenAIRE |
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