Coordination of five class III peroxidase-encoding genes for early germination events of Arabidopsis thaliana

Autor: Vincent Burlat, Sara Raggi, Aurélie Le Ru, Achraf Mohamed Jemmat, Alain Jauneau, Christophe Dunand, Philippe Ranocha, Simone Ferrari, Maxime Neel
Přispěvatelé: Dynamique et Evolution des Parois cellulaires végétales, Laboratoire de Recherche en Sciences Végétales (LRSV), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Pôle de Biotechnologie Végétale, Plateforme d'imagerie cellulaire, Toulouse Réseau Imagerie-Genotoul ( TRI-Genotoul), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)-Fédération de Recherche Agrobiosciences, Interactions et Biodiversité (FR AIB), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Réseau International des Instituts Pasteur (RIIP), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), ANR-11-IDEX-0002,UNITI,Université Fédérale de Toulouse(2011), ANR-18-CE20-0007,MicroWall,Les assemblages moléculaires de microdomaines pariétaux contrôlent la dynamique des parois végétales(2018)
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Mutant
Cell
Arabidopsis
Abbreviations: Class III peroxidase (CIII Prx)
Plant Science
01 natural sciences
Endosperm
chemistry.chemical_compound
MESH: Genes
Plant
Arabidopsis thaliana
cell wall domain remodeling
micropylar endosperm rupture
food and beverages
micropylar endosperm (MEN)
General Medicine
MESH: Arabidopsis Proteins / metabolism
MESH: Arabidopsis Proteins / genetics
Cell biology
MESH: Peroxidases / metabolism
Xyloglucan
medicine.anatomical_structure
Peroxidases
MESH: Germination / genetics
Multigene Family
MESH: Arabidopsis / genetics
MESH: Arabidopsis / physiology
Cell type
Germination
Biology
reactive oxygen species (ROS)
Genes
Plant
Cell wall
03 medical and health sciences
Class III peroxidase
Testa rupture
Micropylar endosperm rupture
Cell wall domain remodeling
Genetics
medicine
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
testa rupture
Arabidopsis Proteins
MESH: eroxidases / genetics
biology.organism_classification
030104 developmental biology
chemistry
MESH: Multigene Family
Agronomy and Crop Science
010606 plant biology & botany
Zdroj: Plant Science
Plant Science, Elsevier, 2020, 298, pp.110565. ⟨10.1016/j.plantsci.2020.110565⟩
ISSN: 1873-2259
0168-9452
DOI: 10.1016/j.plantsci.2020.110565⟩
Popis: The Class III peroxidases (CIII Prxs) belong to a plant-specific multigene family. Thanks to their double catalytic cycle they can oxidize compounds or release reactive oxygen species (ROS). They are either involved in different cell wall stiffening processes such as lignification and suberization, in cell wall loosening or defense mechanisms. Germination is an important developmental stage requiring specific peroxidase activity. However, little is known about which isoforms are involved. Five CIII Prx encoding genes: AtPrx04, AtPrx16, AtPrx62, AtPrx69, and AtPrx71 were identified from published microarray data mining. Delayed or induced testa and endosperm rupture were observed for the corresponding CIII Prx mutant lines indicating either a gene-specific inducing or repressing role during germination, respectively. Via in situ hybridization AtPrx16, AtPrx62, AtPrx69 and AtPrx71 transcripts were exclusively localized to the micropylar endosperm facing the radicle, and transcriptomic data analysis enabled positioning the five CIII Prxs in a co-expression network enriched in germination, cell wall, cell wall proteins and xyloglucan hits. Evidence were produced showing that the five CIII Prxs were cell wall-targeted proteins and that the micropylar endosperm displayed a complex cell wall domain topochemistry. Finally, we drew a spatio-temporal model highlighting the fine sequential gene expression and the possible involvement of micropylar endosperm cell wall domains to explain the non-redundant cell wall stiffening and loosening functions of the CIII Prxs in a single cell type. We also highlighted the necessity of a peroxidase homeostasis to accurately control the micropylar endosperm cell wall dynamics during Arabidopsis germination events.
Databáze: OpenAIRE
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