Selective and complete blockade of acetylcholine-induced relaxation in rabbit aortic rings by N omega-nitro-L-arginine but not by glybenclamide
| Autor: | Lani L. Longenecker, Dennis B. McNamara, J. A. Bellan, Philip J. Kadowitz |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
Cromakalim Muscle Relaxation Vasodilator Agents Pharmacology In Vitro Techniques Arginine Nitroarginine Muscle Smooth Vascular Nitric oxide chemistry.chemical_compound Nitroglycerin medicine.artery Glyburide medicine Animals Benzopyrans Pyrroles Aorta Abdominal Aorta Endothelium-derived relaxing factor Potassium channel Acetylcholine chemistry Anesthesia cardiovascular system Endothelium Vascular Rabbits medicine.symptom medicine.drug Muscle contraction |
| Zdroj: | European journal of pharmacology. 234(2-3) |
| ISSN: | 0014-2999 |
| Popis: | This study addressed the possibility that acetylcholine-induced relaxation in the rabbit aorta is mediated by dual mechanisms: one N omega-nitro-L-arginine (NLA)-sensitive, the other glybenclamide-sensitive. Acetylcholine, nitroglycerin and BRL38227 (lemakalim), an activator of glybenclamide-sensitive potassium channels, were added to an organ bath containing rabbit aortic rings in a cumulative manner in the absence or presence of NLA and/or glybenclamide. NLA inhibited acetylcholine-induced relaxation and potentiated the relaxant response to nitroglycerin. BRL38227 caused a dose-dependent relaxation in rabbit aortic rings, and 30 microM glybenclamide produced essentially complete inhibition of this relaxation. Glybenclamide alone produced no inhibition of acetylcholine-induced relaxation. These results indicate that glybenclamide-sensitive potassium channels in the rabbit aorta play no role in mediating the relaxant response to acetylcholine, while NLA can produce a selective and essentially complete blockade of the relaxant response to acetylcholine in the rabbit aorta. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|