The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase‐2 complex
| Autor: | Frank Sargent, Marta Albareda, Alexander J. Finney, Sarah J. Coulthurst, Michal Fleszar, Rebecca Lowden |
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| Rok vydání: | 2019 |
| Předmět: |
Formates
Pectobacterium Formate dehydrogenase Microbiology Isozyme 03 medical and health sciences chemistry.chemical_compound Hydrogenase Multienzyme Complexes Formate Anaerobiosis Molecular Biology Gene Pectobacterium atrosepticum Research Articles 030304 developmental biology 0303 health sciences biology 030306 microbiology NADH dehydrogenase NADH Dehydrogenase Plants biology.organism_classification Formate Dehydrogenases Biochemistry chemistry biology.protein Oxidation-Reduction Bacteria Function (biology) Research Article Hydrogen |
| Zdroj: | Molecular Microbiology |
| ISSN: | 1365-2958 0950-382X |
| Popis: | Summary Pectobacterium atrosepticum SCRI1043 is a phytopathogenic Gram‐negative enterobacterium. Genomic analysis has identified that genes required for both respiration and fermentation are expressed under anaerobic conditions. One set of anaerobically expressed genes is predicted to encode an important but poorly understood membrane‐bound enzyme termed formate hydrogenlyase‐2 (FHL‐2), which has fascinating evolutionary links to the mitochondrial NADH dehydrogenase (Complex I). In this work, molecular genetic and biochemical approaches were taken to establish that FHL‐2 is fully functional in P. atrosepticum and is the major source of molecular hydrogen gas generated by this bacterium. The FHL‐2 complex was shown to comprise a rare example of an active [NiFe]‐hydrogenase‐4 (Hyd‐4) isoenzyme, itself linked to an unusual selenium‐free formate dehydrogenase in the final complex. In addition, further genetic dissection of the genes encoding the predicted membrane arm of FHL‐2 established surprisingly that the majority of genes encoding this domain are not required for physiological hydrogen production activity. Overall, this study presents P. atrosepticum as a new model bacterial system for understanding anaerobic formate and hydrogen metabolism in general, and FHL‐2 function and structure in particular. Pectobacterium atrospecticum contains the genes for formate hydrogenlyase‐2, considered the ancient progenitor of Complex I. Here, P. atrosepticum was harnessed as a new model system for advancing new knowledge in FHL‐2. The complex was found to contain an unusual selenium‐free formate dehydrogenase and a [NiFe]‐hydrogenase‐4 with a large membrane arm. FHL‐2 was established as the major source of hydrogen gas; however, some components of the membrane arm were surprisingly not essential for this activity. |
| Databáze: | OpenAIRE |
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