Photoaffinity labeling studies of the carbohydrate-binding proteins with different affinities
| Autor: | Tamayo Yamaguchi, Kaori Sakurai, Shimpei Ozawa |
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| Rok vydání: | 2015 |
| Předmět: |
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Molecular Peanut agglutinin Arachis Clinical Biochemistry Pharmaceutical Science Photoaffinity Labels macromolecular substances Plasma protein binding Biochemistry DNA-binding protein Peanut Agglutinin Lectins Drug Discovery Molecular Biology Erythrina biology Photoaffinity labeling Chemistry Organic Chemistry Lectin Castor Bean Affinities biology.protein Carbohydrate Metabolism Molecular Medicine Plant Lectins Selectivity Protein Binding |
| Zdroj: | Bioorganic & Medicinal Chemistry. 23:5319-5325 |
| ISSN: | 0968-0896 |
| DOI: | 10.1016/j.bmc.2015.07.065 |
| Popis: | Photoaffinity labeling has been used as a promising approach to detection and isolation of carbohydrate-binding proteins, which are typically characterized by low binding affinity and selectivity. When there are several specific binding proteins, it is desirable that a photoaffinity probe is capable of simultaneously crosslinking them and that the crosslinking yields depend on the relative binding affinities. In this study, we describe the design and synthesis of carbohydrate photoaffinity probes and their ability to capture lectins of different binding affinities. |
| Databáze: | OpenAIRE |
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