A thioredoxin-like protein of Bemisia tabaci interacts with coat protein of begomoviruses
Autor: | Raman Rajagopal, Vipin Singh Rana, Gunjan Kumar Saurav, Guisuibou Daimei, Sonam Popli |
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Rok vydání: | 2019 |
Předmět: |
Virus transmission
India Whitefly Coat protein Virus Hemiptera 03 medical and health sciences Thioredoxins Solanum lycopersicum Virology Complementary DNA Genetics Animals Molecular Biology Plant Diseases 030304 developmental biology 0303 health sciences biology 030306 microbiology fungi food and beverages General Medicine THIOREDOXIN-LIKE biology.organism_classification Yeast Insect Vectors Begomovirus Vector (epidemiology) Host-Pathogen Interactions Capsid Proteins |
Zdroj: | Virus Genes. 55:356-367 |
ISSN: | 1572-994X 0920-8569 |
DOI: | 10.1007/s11262-019-01657-z |
Popis: | Bemisia tabaci (whitefly) is the sole vector of begomoviruses, which transmits them in a persistent and circulative manner from infected to healthy plants. During this process, begomoviruses interact with various proteins in the insect vector B. tabaci that would play a specific role in the virus transmission. Identification and characterization of such proteins are important to understand the complete process of virus transmission. Coat protein (CP) of begomoviruses is the only protein which is reported to interact with proteins of the insect vector B. tabaci. In this study, we performed yeast two-hybrid assay using CP of cotton leaf curl Rajasthan virus (CLCuV) and Tomato leaf curl New Delhi virus (ToLCNDV) as bait in separate experiments and cDNA prepared from total RNA of B. tabaci was used as prey. Yeast two-hybrid assay resulted in identification of a thioredoxin-like protein (TLP) from CLCuV yeast two-hybrid library. Later TLP was also found to interact with CP of ToLCNDV. In vitro pull-down assay showed TLP interaction with CP of both CLCuV and ToLCNDV. TLP was found to interact with ToLCNDV virus particles isolated from tomato leaves. |
Databáze: | OpenAIRE |
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