Preservation of Tissue-Fixed Immunoglobulins in Skin Biopsies of Patients with Lupus Erythematosus and Bullous DISEASES—Preliminary Report
| Autor: | Yoram Milner, Beno Michel, Kathy David |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Pathology
medicine.medical_specialty Biopsy Immunoglobulins Dermatology Biochemistry Maleimides Magnesium Sulfate Blister Lupus Erythematosus Discoid medicine Humans Lupus Erythematosus Systemic Molecular Biology Fixative Skin Fixation (histology) Frozen section procedure Lupus erythematosus biology medicine.diagnostic_test Tissue Preservation business.industry Cell Biology medicine.disease Pemphigus Ammonium Sulfate biology.protein Antibody business |
| Zdroj: | Journal of Investigative Dermatology. 59:449-452 |
| ISSN: | 0022-202X |
| DOI: | 10.1111/1523-1747.ep12627611 |
| Popis: | Demonstration of tissue-fixed immunoglobulins in skin biopsies in a useful diagnostic procedure in lupus erythematosus and bullous diseases. The main limitation of the procedure has been the need for frozen tissue. A solution containing ammonium sulfate, N-ethyl maleimide, and magnesium sulfate in citrate buffer has been developed for the preservation of tissue-fixed immunoglobulins. Preserved specimens can be mailed to laboratories and the need for frozen sections is removed in most cases. Matched frozen and fixed specimens from 13 patients yielded comparable results. Freezing and thawing before fixation resulted in decreased or complete loss of fluorescence. Fixed sections could also be stained with horseradish peroxidase-labelled immunoglobulins with good results. The remaining tissue could be fixed subsequently in 10% formalin for routine histologic examination with satisfactory results. The fixative should make the demonstration of tissue-fixed immunoglobulins a readily available clinical test. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|