Field-based performance of three pre-market rapid hepatitis C virus antibody assays in STAHR (Study to Assess Hepatitis C Risk) among young adults who inject drugs in San Diego, CA
| Autor: | Cindy M. Weinbaum, Eyasu H. Teshale, Bryce D. Smith, Amy Jewett, Richard S. Garfein, Jazmine Cuevas-Mota |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adult
Male medicine.medical_specialty Time Factors Multivariate analysis Adolescent Point-of-Care Systems Hepacivirus Hepatitis C virus Hepatitis C virus Antibody medicine.disease_cause Sensitivity and Specificity Article Young Adult Virology Internal medicine medicine Humans Field based Young adult Substance Abuse Intravenous Immunoassay medicine.diagnostic_test biology Clinical Laboratory Techniques business.industry Hepatitis C Hepatitis C Antibodies medicine.disease biology.organism_classification United States Infectious Diseases Immunology Female business |
| Zdroj: | Journal of Clinical Virology. 54:213-217 |
| ISSN: | 1386-6532 |
| DOI: | 10.1016/j.jcv.2012.04.003 |
| Popis: | Background Approximately 4.1 million Americans are estimated to have been infected with hepatitis C virus (HCV), 45–85% of whom are unaware of their infection. Persons who inject drugs (PWID) account for 55.8% of all persons with HCV antibody (anti-HCV) in the U.S. PWID have limited access to healthcare and are infrequently tested for anti-HCV using conventional laboratory assays. Objective To evaluate performance characteristics (sensitivity and specificity) of three, pre-market rapid point-of-care tests (one oral fluid and two finger-stick assays) from two manufacturers (Chembio and MedMira) in settings providing services to young adult PWID in San Diego, CA. Study design Behavioral risk assessment surveys and testing for HCV were conducted among persons who reported injection drug use (IDU) within the past 6 months as part of the Study to Assess Hepatitis C Risk (STAHR) among PWID aged 18–40 years in 2009–2010. Sensitivity and specificity of the rapid anti-HCV assays were evaluated among STAHR participants, using two commonly used testing algorithms. Results Variability in sensitivity (76.6–97.1%) and specificity (99.0–100.0%) was found across assays. The highest sensitivity achieved for the Chembio finger-stick blood, Chembio oral fluid and MedMira finger-stick blood tests was 97.1%, 85.4% and 80.0% respectively; the highest specificity was 99.0%, 100.0% and 100.0%, respectively. In multivariate analysis false negative anti-HCV results were associated with female sex for the MedMira blood assay. Conclusions Sensitive anti-HCV rapid assays are appropriate and feasible for high-prevalence, high-risk populations such as young PWID. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect