Single-cell analysis defines the divergence between the innate lymphoid cell lineage and lymphoid tissue–inducer cell lineage

Autor: Herman Gudjonson, Rachel Golub, Sylvestre Chea, Aaron R. Dinner, Michael G. Constantinides, Isabel E. Ishizuka, Albert Bendelac
Přispěvatelé: Committee on Immunology, University of Chicago, Department of pathology, Lymphopoïèse (Lymphopoïèse (UMR_1223 / U1223 / U-Pasteur_4)), Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Cellule Pasteur, Université Paris Diderot - Paris 7 (UPD7)-PRES Sorbonne Paris Cité, institute of biophysical dynamics, department of biochemistry, Lymphopoïèse, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Supported by the US National Institutes of Health (R01 HL118092, AI038339 and AI108643) and by the Digestive Diseases Research Center of Excellence (P30DK42086), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Rok vydání: 2016
Předmět:
Zdroj: Nature Immunology
Nature Immunology, Nature Publishing Group, 2016, 17 (3), pp.269-76. ⟨10.1038/ni.3344⟩
Nature Immunology, 2016, 17 (3), pp.269-76. ⟨10.1038/ni.3344⟩
ISSN: 1529-2916
1529-2908
DOI: 10.1038/ni.3344
Popis: International audience; The precise lineage relationship between innate lymphoid cells (ILCs) and lymphoid tissue-inducer (LTi) cells is poorly understood. Using single-cell multiplex transcriptional analysis of 100 lymphoid genes and single-cell cultures of fetal liver precursor cells, we identified the common proximal precursor to these lineages and found that its bifurcation was marked by differential induction of the transcription factors PLZF and TCF1. Acquisition of individual effector programs specific to the ILC subsets ILC1, ILC2 and ILC3 was initiated later, at the common ILC precursor stage, by transient expression of mixed ILC1, ILC2 and ILC3 transcriptional patterns, whereas, in contrast, the development of LTi cells did not go through multilineage priming. Our findings provide insight into the divergent mechanisms of the differentiation of the ILC lineage and LTi cell lineage and establish a high-resolution 'blueprint' of their development.
Databáze: OpenAIRE
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