The influence of Peyer's patch apoptosis on intestinal mucosal immunity in burned mice
| Autor: | Yong Xie, Guanghua Guo, Wei Feng, Jun Fan, Xuedong Li, Qingyan Meng, Yiping Xiu, Liang Ma, Tairan Li |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Immunoglobulin A
Male Ratón CD3 Apoptosis Critical Care and Intensive Care Medicine Flow cytometry Immunophenotyping Andrology chemistry.chemical_compound Mice Peyer's Patches T-Lymphocyte Subsets Intestine Small Medicine Animals Propidium iodide Intestinal Mucosa Immunity Mucosal B-Lymphocytes Mice Inbred BALB C medicine.diagnostic_test biology business.industry Peyer's patch General Medicine medicine.anatomical_structure chemistry Immunology Emergency Medicine biology.protein Surgery business Burns CD8 |
| Zdroj: | Burns : journal of the International Society for Burn Injuries. 35(5) |
| ISSN: | 1879-1409 |
| Popis: | The aim of this study was to investigate the influence of apoptosis on Peyer's patches and the intestinal immunoglobulin A (IgA) response in burned mice. Sixty male Balb/c mice were randomly assigned into the sham-burn (control) group (n=30) and the burn group (n=30). The mice in the burn group received a full-thickness scald burn over 20% of the total body surface area (TBSA), on the back. At 12, 24 and 72 h, respectively, after injury, the burned mice (n=10, at every time point) were anaesthetised and their entire intestines were collected. The mice in the sham-burn group were treated with the same procedure as above, except for the burn injury. The number of Peyer's patches on every entire intestine and the total Peyer's patches cell yield were counted. The changes of lymphocyte subpopulations in Peyer's patches were measured by flow cytometry (FCM). And the levels of intestinal IgA were examined by enzyme-linked immunosorbent assay (ELISA). Fluoresceinisothiocyanate (FITC)-conjugated Annexin-tau and propidium iodide (PI) double-staining cells were analysed by FCM for apoptotic ratio in Peyer's patches. The results showed that the total Peyer's patch cell yield and the numbers of CD3, CD4, CD8 and CD19 cells were significantly decreased at 12, 24 and 72 h after injury (P |
| Databáze: | OpenAIRE |
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