Time-course study of SCG10 mRNA levels associated with LTP induction and maintenance in the rat Schaffer-CA1 pathway in vivo
| Autor: | Haixiang Peng, Joe L. Martinez, Brian E. Derrick |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Time Factors Neurite Long-Term Potentiation Hippocampus In situ hybridization Hippocampal formation Biology Functional Laterality Piperazines Cellular and Molecular Neuroscience Neural Pathways LTP induction Animals Nerve Growth Factors RNA Messenger Molecular Biology In Situ Hybridization Neurons Reverse Transcriptase Polymerase Chain Reaction musculoskeletal neural and ocular physiology Dentate gyrus Membrane Proteins Long-term potentiation Electric Stimulation Rats Inbred F344 Rats nervous system Synaptic plasticity Microtubule Proteins Autoradiography Carrier Proteins Excitatory Amino Acid Antagonists Neuroscience |
| Zdroj: | Molecular Brain Research. 120:182-187 |
| ISSN: | 0169-328X |
| Popis: | The maintenance of long-term potentiation (LTP) depends on altered gene expression. Previously, we found the expression of neuronal growth associated protein SCG10, which is involved in neurite outgrowth and neural regeneration, was up-regulated by LTP induction in the rat hippocampal Schaffer-collateral CA1 pathway. Here we studied the temporal expression pattern of SCG10 mRNA after LTP induction using permanently implanted electrodes in the same CA1 pathway. The real-time RT-PCR showed that both SCG10 mRNA 1 and 2 kb forms were increased at the 3 h, but not at 1 or 24 h. In situ hybridization revealed an increase of SCG10 2 kb mRNA level in ipsilateral CA3 and CA1 areas, but not their contralateral counterparts or either side of dentate gyrus. These results suggest that SCG10 may play a role in the maintenance of synaptic plasticity through a transient regulation of microtubule dynamics, which facilitates the structural remodeling of the presynaptic element during the consolidation period. |
| Databáze: | OpenAIRE |
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